Selective removal of the autoantibodies from rheumatoid arthritis patient plasma using protein A carrying affinity cryogels

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dc.contributor.authorAlkan, Huseyin
dc.contributor.authorBereli, Nilay
dc.contributor.authorBaysal, Zubeyde
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-04-24T16:10:54Z
dc.date.available2024-04-24T16:10:54Z
dc.date.issued2010
dc.departmentDicle Üniversitesien_US
dc.description.abstractRheumatoid arthritis is a chronic, progressive, deabilitating autoimmune disease that occurs in approximately 1% of adults. Rheumatoid arthritis is characterized by chronic polyarthritis and destruction of multiple joints. In this study, IgM-antibody removal from human plasma with supermacroporous poly(hydroxyethyl methacrylate) [PHEMA] cryogel carrying protein A has been evaluated. The PHEMA cryogel was prepared by bulk polymerization which proceeds in an aqueous solution of monomer frozen inside a plastic syringe (cryo-polymerization). After thawing, the PHEMA cryogel contains a continuous matrix having interconnected macropores of 10-200 mu m size. Pore volume in the PHEMA cryogel was 71.6%. Protein A molecules were covalently immobilized onto the PHEMA cryogel via cyanogen bromide (CNBr) activation. The PHEMA cryogel was contacted with blood in in vitro system for the determination of blood-compatibility. The supermacroporous structure of the PHEMA cryogel makes it possible to process blood cells without blocking the cryogel column. IgM-antibody adsorption capacity decreased significantly with the increase of the plasma flow-rate. The maximum IgM-antibody adsorption amount was 42.7 mg/g. IgM-antibody molecules could be repeatedly adsorbed and eluted without noticeable loss in the IgM-antibody adsorption amount. (C) 2010 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.bej.2010.06.010
dc.identifier.endpage159en_US
dc.identifier.issn1369-703X
dc.identifier.issn1873-295X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-77955419442
dc.identifier.scopusqualityQ2
dc.identifier.startpage153en_US
dc.identifier.urihttps://doi.org/10.1016/j.bej.2010.06.010
dc.identifier.urihttps://hdl.handle.net/11468/15179
dc.identifier.volume51en_US
dc.identifier.wosWOS:000281936900009
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRheumatoid Arthritisen_US
dc.subjectPhemaen_US
dc.subjectAntibody Removalen_US
dc.subjectAffinity Therapyen_US
dc.subjectCryogelsen_US
dc.titleSelective removal of the autoantibodies from rheumatoid arthritis patient plasma using protein A carrying affinity cryogelsen_US
dc.titleSelective removal of the autoantibodies from rheumatoid arthritis patient plasma using protein A carrying affinity cryogels
dc.typeArticleen_US

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