Immobilization of ?-amylase via adsorption onto bentonite/chitosan composite: Determination of equilibrium, kinetics and thermodynamic parameters

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dc.contributor.authorBaysal, Zübeyde
dc.contributor.authorBulut, Yasemin
dc.contributor.authorYavuz, Murat
dc.contributor.authorAytekin, Çetin
dc.date.accessioned2024-04-24T15:59:33Z
dc.date.available2024-04-24T15:59:33Z
dc.date.issued2014
dc.departmentDicle Üniversitesi, Fen Fakültesi, Kimya Bölümüen_US
dc.description.abstractImmobilization of -amylase onto bentonite/chitosan (BC) composite was studied via adsorption. The composite was characterized by FTIR, SEM, and surface area measurements. The effect of different factors such as, pH, temperature, initial enzyme concentration, and various thermodynamic parameters was determined. The maximum -amylase adsorption capacity of the BC composite was determined as 64mg/g at 0.8mg/mL enzyme concentration. The activity of the immobilized enzyme was measured under varying experimental conditions. The highest enzyme activity for free and immobilized enzyme was determined at 30 and 35 degrees C in 0.1M phosphate buffer at pH 7.0. The effect of substrate concentration on enzyme activity of free and immobilized enzymes showed a good fit to the Lineweaver-Burk plots. Michaelis constant, K-m, for the immobilized -amylase was found to be higher than for the free enzyme. The adsorption isotherm was modeled by the Langmuir equation.en_US
dc.description.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1002/star.201300133
dc.identifier.citationBaysal, Z., Bulut, Y., Yavuz, M. ve Aytekin, C. (2014). Immobilization of α-amylase via adsorption onto bentonite/ chitosan composite: Determination of equilibrium, kinetics and thermodynamic parameters. Starch/Staerke, 66(5-6), 484–490.
dc.identifier.doi10.1002/star.201300133
dc.identifier.endpage490en_US
dc.identifier.issn0038-9056
dc.identifier.issn1521-379X
dc.identifier.issue5-6en_US
dc.identifier.scopus2-s2.0-84901608114
dc.identifier.scopusqualityQ2
dc.identifier.startpage484en_US
dc.identifier.urihttps://doi.org/10.1002/star.201300133
dc.identifier.urihttps://hdl.handle.net/11468/14137
dc.identifier.volume66en_US
dc.identifier.wosWOS:000334925000009
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorBaysal, Zübeyde
dc.institutionauthorBulut, Yasemin
dc.institutionauthorYavuz, Murat
dc.institutionauthorAytekin, Çetin
dc.language.isoenen_US
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofStarch-Starke
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject-Amylaseen_US
dc.subjectBentoniteen_US
dc.subjectChitosanen_US
dc.subjectCompositeen_US
dc.subjectImmobilizationen_US
dc.titleImmobilization of ?-amylase via adsorption onto bentonite/chitosan composite: Determination of equilibrium, kinetics and thermodynamic parametersen_US
dc.titleImmobilization of ?-amylase via adsorption onto bentonite/chitosan composite: Determination of equilibrium, kinetics and thermodynamic parameters
dc.typeArticleen_US

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