Phytochemical analysis, and antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex L. and Pinus halepensis Mill. methanolic extracts

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dc.contributor.authorMeziti, Hicham
dc.contributor.authorBouriche, Hamama
dc.contributor.authorKada, Seoussen
dc.contributor.authorDemirtas, Ibrahim
dc.contributor.authorKizil, Murat
dc.contributor.authorSenator, Abderrahmane
dc.date.accessioned2024-04-24T17:37:45Z
dc.date.available2024-04-24T17:37:45Z
dc.date.issued2019
dc.departmentDicle Üniversitesien_US
dc.description.abstractContext: Quercus ilex and Pinus halepensis are largely used in Algerian folk medicine as a remedy for different health problems. Aims: To determine the phytochemical composition as well as to evaluate the antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex root bark extract (QIE) and Pinus halepensis young ovulate cones extract (PHE). Methods: Methanolic extracts were prepared by maceration. Phenolic compounds and flavonoids were quantified spectrophotometrically and identified by HPLC analysis. In vitro antioxidant activity of the extracts was assessed by determining the DPPH and ABTS radicals scavenging activities and ferric reducing/antioxidant power (FRAP). Protective effect of the extracts against AAPH-induced erythrocyte hemolysis was assessed. pBluescript M13 (+) plasmid DNA was used as oxidation target to evaluate DNA protective effect of the extracts. Results: Considerable phenolic compounds and flavonoids contents were found in the studied extracts. Catechin and phenolic acids (4-hydoxybenzoic, caffeic, coumaric, ferulic and gentisic acids) were identified in QIE, while catechin and cinnamic acid were identified in PHE. Both extracts scavenge DPPH radical with IC50 values of 5.67 mu g/mL and 18.87 mu g/mL, respectively. Antioxidant capacity against ABTS radical was 4.13 and 1.15 mM Trolox equivalent/mg extract. The two extracts also showed a considerable ferric reducing ability with 1.98 and 8.55 mM FeSO4/mg extract, respectively. On the other hand, both extracts exerted a significant protective effect against AAPH-induced erythrocyte hemolysis and DNA protective activities were noticed. Conclusions: Quercus ilex and Pinus halepensis extracts are important sources of bioactive compounds possessing important antioxidants, antihemolytic and genoprotective effects.en_US
dc.description.sponsorshipAlgerian Ministry of Higher Education and Scientific Research [CNEPRU/D01N01UN190120150001]en_US
dc.description.sponsorshipThis work was supported by the Algerian Ministry of Higher Education and Scientific Research. Project Ref: CNEPRU/D01N01UN190120150001.en_US
dc.identifier.endpage272en_US
dc.identifier.issn0719-4250
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85071941576
dc.identifier.scopusqualityQ1
dc.identifier.startpage260en_US
dc.identifier.urihttps://hdl.handle.net/11468/21165
dc.identifier.volume7en_US
dc.identifier.wosWOS:000473339300004
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherJournal Pharmacy & Pharmacognosy Research-Jppresen_US
dc.relation.ispartofJournal of Pharmacy & Pharmacognosy Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnti-Hemolyticen_US
dc.subjectAntioxidanten_US
dc.subjectGenoprotectionen_US
dc.subjectPinus Halepensisen_US
dc.subjectQuercus Ilexen_US
dc.titlePhytochemical analysis, and antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex L. and Pinus halepensis Mill. methanolic extractsen_US
dc.titlePhytochemical analysis, and antioxidant, anti-hemolytic and genoprotective effects of Quercus ilex L. and Pinus halepensis Mill. methanolic extracts
dc.typeArticleen_US

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