Optimization of the thermostable alkaline and Ca-dependent ?-amylase production from Bacillus paralicheniformis by statistical modeling

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dc.authorid0000-0001-8253-9568en_US
dc.authorid0000-0001-7283-9225en_US
dc.authorid0000-0001-9438-5447en_US
dc.contributor.authorBekler, Fatma Matpan
dc.contributor.authorYalaz, Seçi̇l
dc.contributor.authorGüven, Reyhan Gül
dc.contributor.authorGüven, Kemal
dc.date.accessioned2021-09-29T05:53:10Z
dc.date.available2021-09-29T05:53:10Z
dc.date.issued2019en_US
dc.departmentDicle Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Ana Bilim Dalıen_US
dc.description.abstractA novel amylolytic enzyme producing thermoalkaliphilic bacterium, the source of industrially used enzymes was isolated. Isolated strain was identified by morphological, physio-biochemical tests and the 16S rRNA gene sequence analysis. The optimal conditions of enzyme activity were determined. For higher α-amylase production, the variables such as yeast extract, starch, CaCl2, (NH4)2SO4, NaCl and MgSO4 in the α-amylase production medium, the temperature and pH were screened by Plackett–Burman design and optimised using response surface methodology (RSM). The optimal conditions were found to be 0.15 g/L for starch, 0.15 mg/L for CaCl2 and 60 °C for temperature. By using RSM model, amylase production increase was achieved sevenfold. It is showed that this method can be utilised to optimize α-amylase production in athermophilic bacteria such as Bacillus paralicheniformis. Keywords: α-amylase; Bacillus paralicheniformis; optimization; response surface methodology.en_US
dc.identifier.citationBekler, F. M., Yalaz, S., Güven, R. G. ve Güven, K. (2019). Optimization of the thermostable alkaline and Ca-dependent α-amylase production from Bacillus paralicheniformis by statistical modeling. Journal of the Serbian Chemical Society, 84(10), 1093-1104.en_US
dc.identifier.doi10.2298/JSC190227021B
dc.identifier.endpage1104en_US
dc.identifier.issn0352-5139
dc.identifier.issue10en_US
dc.identifier.scopus2-s2.0-85074889815
dc.identifier.scopusqualityQ3
dc.identifier.startpage1093en_US
dc.identifier.urihttp://www.doiserbia.nb.rs/Article.aspx?ID=0352-51391900021B
dc.identifier.urihttps://hdl.handle.net/11468/7810
dc.identifier.volume84en_US
dc.identifier.wosWOS:000496149600004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorBekler, Fatma Matpan
dc.institutionauthorYalaz, Seçi̇l
dc.institutionauthorGüven, Reyhan Gül
dc.institutionauthorGüven, Kemal
dc.language.isoenen_US
dc.publisherSerbian Chemical Societyen_US
dc.relation.ispartofJournal of the Serbian Chemical Society
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectα-Amylaseen_US
dc.subjectBacillus paralicheniformisen_US
dc.subjectOptimizationen_US
dc.subjectResponse surface methodologyen_US
dc.titleOptimization of the thermostable alkaline and Ca-dependent ?-amylase production from Bacillus paralicheniformis by statistical modelingen_US
dc.titleOptimization of the thermostable alkaline and Ca-dependent ?-amylase production from Bacillus paralicheniformis by statistical modeling
dc.typeArticleen_US

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