Optimization of the thermostable alkaline and Ca-dependent ?-amylase production from Bacillus paralicheniformis by statistical modeling
dc.authorid | 0000-0001-8253-9568 | en_US |
dc.authorid | 0000-0001-7283-9225 | en_US |
dc.authorid | 0000-0001-9438-5447 | en_US |
dc.contributor.author | Bekler, Fatma Matpan | |
dc.contributor.author | Yalaz, Seçi̇l | |
dc.contributor.author | Güven, Reyhan Gül | |
dc.contributor.author | Güven, Kemal | |
dc.date.accessioned | 2021-09-29T05:53:10Z | |
dc.date.available | 2021-09-29T05:53:10Z | |
dc.date.issued | 2019 | en_US |
dc.department | Dicle Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Ana Bilim Dalı | en_US |
dc.description.abstract | A novel amylolytic enzyme producing thermoalkaliphilic bacterium, the source of industrially used enzymes was isolated. Isolated strain was identified by morphological, physio-biochemical tests and the 16S rRNA gene sequence analysis. The optimal conditions of enzyme activity were determined. For higher α-amylase production, the variables such as yeast extract, starch, CaCl2, (NH4)2SO4, NaCl and MgSO4 in the α-amylase production medium, the temperature and pH were screened by Plackett–Burman design and optimised using response surface methodology (RSM). The optimal conditions were found to be 0.15 g/L for starch, 0.15 mg/L for CaCl2 and 60 °C for temperature. By using RSM model, amylase production increase was achieved sevenfold. It is showed that this method can be utilised to optimize α-amylase production in athermophilic bacteria such as Bacillus paralicheniformis. Keywords: α-amylase; Bacillus paralicheniformis; optimization; response surface methodology. | en_US |
dc.identifier.citation | Bekler, F. M., Yalaz, S., Güven, R. G. ve Güven, K. (2019). Optimization of the thermostable alkaline and Ca-dependent α-amylase production from Bacillus paralicheniformis by statistical modeling. Journal of the Serbian Chemical Society, 84(10), 1093-1104. | en_US |
dc.identifier.doi | 10.2298/JSC190227021B | |
dc.identifier.endpage | 1104 | en_US |
dc.identifier.issn | 0352-5139 | |
dc.identifier.issue | 10 | en_US |
dc.identifier.scopus | 2-s2.0-85074889815 | |
dc.identifier.scopusquality | Q3 | |
dc.identifier.startpage | 1093 | en_US |
dc.identifier.uri | http://www.doiserbia.nb.rs/Article.aspx?ID=0352-51391900021B | |
dc.identifier.uri | https://hdl.handle.net/11468/7810 | |
dc.identifier.volume | 84 | en_US |
dc.identifier.wos | WOS:000496149600004 | |
dc.identifier.wosquality | Q4 | |
dc.indekslendigikaynak | Web of Science | |
dc.indekslendigikaynak | Scopus | |
dc.institutionauthor | Bekler, Fatma Matpan | |
dc.institutionauthor | Yalaz, Seçi̇l | |
dc.institutionauthor | Güven, Reyhan Gül | |
dc.institutionauthor | Güven, Kemal | |
dc.language.iso | en | en_US |
dc.publisher | Serbian Chemical Society | en_US |
dc.relation.ispartof | Journal of the Serbian Chemical Society | |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | α-Amylase | en_US |
dc.subject | Bacillus paralicheniformis | en_US |
dc.subject | Optimization | en_US |
dc.subject | Response surface methodology | en_US |
dc.title | Optimization of the thermostable alkaline and Ca-dependent ?-amylase production from Bacillus paralicheniformis by statistical modeling | en_US |
dc.title | Optimization of the thermostable alkaline and Ca-dependent ?-amylase production from Bacillus paralicheniformis by statistical modeling | |
dc.type | Article | en_US |
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