Effect of Cytokinins on In Vitro Propagation of Ajuga xylorrhiza Kit Tan (Critically Endangered), Endemic to Turkey

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dc.contributor.authorIsikalan, Cidem
dc.contributor.authorOrcan, Pinar
dc.contributor.authorAkbas, Filiz
dc.contributor.authorNamli, Sureyya
dc.contributor.authorKuru, Ibrahim Selcuk
dc.contributor.authorBulus, Serife
dc.date.accessioned2024-04-24T16:02:29Z
dc.date.available2024-04-24T16:02:29Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractAjuga xylorrhiza Kit Tan is a rare endemic perennial species growing in the stony habitats in Southeastern Anatolia region, Turkey. An efficient and reproducible protocol has been developed for micropropagation of endangered Ajuga xylorrhiza. Shoots were cultured on Murashige and Skoog (MS) basal medium, supplemented with various concentrations of 6-benzyl amino purine (BAP), thidiazuron (TDZ), and kinetin (Kin). The optimal shoot formation was with the medium containing 0.125 mg L-1 BAP (7.91 shoots per explant), and 0.25 mg L-1 Kin (4.9 shoots per explant) was recorded. The best result in terms of shoot length (3.43 to 3.63 cm) was obtained from the Kin treatments. Kin parameters were more effective on shoot length and multiple shoot production than BAP and TDZ. For rooting, regenerated shoots were transferred to 1/4 strength MS medium containing two different concentrations of indole acetic acid (IAA) and alpha-naphthalenetic acid (NAA) at 0 (control), 0.5, or 1.0 mg L-1.The best results in terms of the number of roots per shoot were obtained from the medium supplemented with 0.5 mg L-1 IAA (3.10 roots per explant). Rooted plantlets were transferred to soil for acclimization with 100% success rate.en_US
dc.description.sponsorshipBatman University Scientific Research Unit [BTUBAP-2012-FED11]en_US
dc.description.sponsorshipThe authors would like to thank the Batman University Scientific Research Unit (Project No.: BTUBAP-2012-FED11) for the financial support for a part of this study.en_US
dc.identifier.doi10.1007/s11627-020-10082-z
dc.identifier.endpage914en_US
dc.identifier.issn1054-5476
dc.identifier.issn1475-2689
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85085287411
dc.identifier.scopusqualityQ1
dc.identifier.startpage911en_US
dc.identifier.urihttps://doi.org/10.1007/s11627-020-10082-z
dc.identifier.urihttps://hdl.handle.net/11468/14812
dc.identifier.volume56en_US
dc.identifier.wosWOS:000534495500001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-Plant
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAjuga Xylorrhiza Kit Tanen_US
dc.subjectEndemicen_US
dc.subjectCritically Endangereden_US
dc.subjectMicropropagationen_US
dc.subjectCytokininen_US
dc.titleEffect of Cytokinins on In Vitro Propagation of Ajuga xylorrhiza Kit Tan (Critically Endangered), Endemic to Turkeyen_US
dc.titleEffect of Cytokinins on In Vitro Propagation of Ajuga xylorrhiza Kit Tan (Critically Endangered), Endemic to Turkey
dc.typeArticleen_US

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