Characterization of intracellular β-galactosidase from Bacillus subtilis 4NK and Bacillus paralicheniformis 5NK isolated from a hot water spring and effects of various inhibitors on enzyme activity

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dc.contributor.authorTunç, Şaban
dc.contributor.authorBekler, Fatma Matpan
dc.contributor.authorGüven, Kemal
dc.date.accessioned2024-04-24T17:58:16Z
dc.date.available2024-04-24T17:58:16Z
dc.date.issued2021
dc.departmentDicle Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.description.abstractIn this study, the intracellular ?-galactosidases of Bacillus subtilis 4NK and Bacillus paralicheniformis 5NK isolated from Bingöl Binkap hot spring was partially purified and characterized. As a result of purification, the yield of the enzyme for B. subtilis 4NK was 85.2% and the purification fold was 2.8. The yield for B. paralicheniformis 5NK was 76.8% and the purification fold was 2.0. The optimum temperature of the enzyme was determined as 45oC for B. subtilis 4NK and 55oC for B. paralicheniformis 5NK and the optimum pH was 6.0 for both. In addition, in the thermal stability experiments even at the end of 120 min both enzymes were stable at 50oC. It was determined that the partially purified enzyme activity increased in the presence of iodoacetamide and phenylmethylsulfonylfluoride for B. subtilis 4NK, dithiothreitol, N-ethylenemaleimide and phenylmethylsulfonylfluoride for B. paralicheniformis 5NK. The metals were found to activate the enzyme at low concentrations of Co2+, Cd2+ and Mn2+ for B. subtilis 4NK, Cu2+ and Cd2+ were found to inhibit the enzyme at high rates for B. paralicheniformis 5NK. Km and Vmax values for 4NK and 5NK, respectively; 23.80 mM, 1.978 µmol/min and 5.61 mM, 1.869 µmol/min.en_US
dc.description.sponsorshipThis study was supported by the projects FEN.17.008 and FEN.17.009 and the Dicle University Scientific Research Projects Coordinator ?DÜB?P ? ?en_US
dc.identifier.citationTunç, Ş., Bekler, F. M. ve Güven, K. (2021). Characterization of intracellular β-galactosidase from Bacillus subtilis 4NK and Bacillus paralicheniformis 5NK isolated from a hot water spring and effects of various inhibitors on enzyme activity. Biotech Studies, 30(2), 71-78.
dc.identifier.doi10.38042/biotechstudies.953514
dc.identifier.endpage78en_US
dc.identifier.issn2687-3761
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85167978584
dc.identifier.scopusqualityQ3
dc.identifier.startpage71en_US
dc.identifier.trdizinidTRDizinIdYok
dc.identifier.urihttps://doi.org/10.38042/biotechstudies.953514
dc.identifier.urihttps://hdl.handle.net/11468/23780
dc.identifier.volume30en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.publisherField Crops Central Research Instituteen_US
dc.relation.ispartofBiotech Studies
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacillus paralicheniformis 5nken_US
dc.subjectBacillus subtilis 4nken_US
dc.subjectβ-galactosidase characterisationen_US
dc.titleCharacterization of intracellular β-galactosidase from Bacillus subtilis 4NK and Bacillus paralicheniformis 5NK isolated from a hot water spring and effects of various inhibitors on enzyme activityen_US
dc.titleCharacterization of intracellular ?-galactosidase from Bacillus subtilis 4NK and Bacillus paralicheniformis 5NK isolated from a hot water spring and effects of various inhibitors on enzyme activity
dc.typeArticleen_US

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