A fast, accurate and comprehensive LC-MS/MS method validation for the sensitive quantification of water-soluble vitamins in walnut, almond, hazelnut and pistachio fruits

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dc.contributor.authorYilmaz, Mustafa Abdullah
dc.contributor.authorCakir, Oguz
dc.contributor.authorYener, Ismail
dc.date.accessioned2024-04-24T16:14:54Z
dc.date.available2024-04-24T16:14:54Z
dc.date.issued2023
dc.departmentDicle Üniversitesien_US
dc.description.abstractEvaluating nutritional values of food products such as nuts has been of great importance nowadays. In the current study, development and validation of a robust and fast LC-MS/MS method was carried out for simultaneous determination of pantothenic acid (B5), riboflavin (B2), biotin (B7), thiamine (B1), cyano-cobalamine (B12), nicotinamide (B3), nicotinic acid (B3), pyridoxine (B6) and folic acid (B9) in walnut, almond, hazelnut and pistachio samples from Turkey. Isotope labeled vitamin standards were used to compensate the sensitivity losses and increase the degree of precision. The validation parameters of the developed analytical method were accuracy (recovery), linearity, limit of quantification (LOQ), intra-day and inter-day precision (repeatability), relative standard uncertainty (U% at 95% confidence level (k 1/4 2)), and limit of detection (LOD). Chromatographic parameters were optimized to have optimum separation of nine water soluble vitamins (WSVs) and MS detection was performed using a tandem mass spec-trometer. Linearity of the established LC-MS/MS method was in the range of 25-1000 ng/mL for the studied vitamins. The coefficients of determination for the vitamins were between 0.9973 and 0.9999. LOD/LOQ values of the analytes were 10.48/12.54, 13.08/19.59, 5.77/7.70, 12.48/17.71, 12.38/14.90, 6.55/ 10.08, 8.08/9.81, 13.03/16.38, 13.08/19.59 for B1, B2, nicotinamide (B3), nicotinic acid (B3), B5, B6, B7, B9 and B12, respectively.(c) 2023 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.ijms.2023.117040
dc.identifier.issn1387-3806
dc.identifier.issn1873-2798
dc.identifier.scopus2-s2.0-85150055054
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.ijms.2023.117040
dc.identifier.urihttps://hdl.handle.net/11468/15503
dc.identifier.volume488en_US
dc.identifier.wosWOS:000955762300001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Mass Spectrometry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLc-Msen_US
dc.subjectMsen_US
dc.subjectMethod Validationen_US
dc.subjectWater Soluble Vitaminsen_US
dc.subjectIsotope Labeled Internal Standarden_US
dc.subjectNutsen_US
dc.titleA fast, accurate and comprehensive LC-MS/MS method validation for the sensitive quantification of water-soluble vitamins in walnut, almond, hazelnut and pistachio fruitsen_US
dc.titleA fast, accurate and comprehensive LC-MS/MS method validation for the sensitive quantification of water-soluble vitamins in walnut, almond, hazelnut and pistachio fruits
dc.typeArticleen_US

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