Unusual genetic organization of a functional type I protein secretion system in Neisseria meningitidis

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dc.contributor.authorWooldridge, Karl G.
dc.contributor.authorKızıl, Murat
dc.contributor.authorWells, Damien B.
dc.contributor.authorAla'Aldeen, Dlawer A. A.
dc.date.accessioned2024-04-24T17:56:30Z
dc.date.available2024-04-24T17:56:30Z
dc.date.issued2005
dc.departmentDicle Üniversitesi, Fen Fakültesi, Kimya Bölümüen_US
dc.description.abstractProteins secreted by Neisseria meningitidis are thought to play important roles in the pathogenesis of meningococcal disease. These proteins include the iron-repressible repeat-in-toxin (RTX) exoprotein FrpC. Related proteins in other pathogens are secreted via a type I secretion system (TOSS), but such a system has not been demonstrated in N. meningitidis. An in silico search of the group B meningococcal genome suggested the presence of a uniquely organized TOSS. Genes encoding homologs of the Escherichia coli HlyB (ATP-binding), HlyD (membrane fusion), and TolC (outer membrane channel) proteins were identified. In contrast to the cistronic organization of the secretion genes in most other rtx operons, the hlyD and tolC genes were adjacent but unlinked to hlyB; neither locus was part of an operon containing genes encoding putative TOSS substrates. Both loci were flanked by genes normally associated with mobile genetic elements. The three genes were shown to be expressed independently. Mutation at either locus resulted in an inability to secrete FrpC and a related protein, here called FrpC2. Successful complementation of these mutations at an ectopic site confirmed the observed phenotypes were caused by loss of function of the putative TOSS genes. We show that genes scattered in the meningococcal genome encode a functional TOSS required for secretion of the meningococcal RTX proteins.en_US
dc.identifier.citationWooldridge, K. G., Kızıl, M., Wells, D. B. ve Ala'Aldeen, D. A. A. (2005). Unusual genetic organization of a functional type I protein secretion system in Neisseria meningitidis. Infection and Immunity, 73(9), 5554-5567.
dc.identifier.doi10.1128/IAI.73.9.5554-5567.2005
dc.identifier.endpage5567en_US
dc.identifier.issn0019-9567
dc.identifier.issue9en_US
dc.identifier.pmid16113272
dc.identifier.scopus2-s2.0-23944505577
dc.identifier.scopusqualityQ1
dc.identifier.startpage5554en_US
dc.identifier.urihttps://doi.org/10.1128/IAI.73.9.5554-5567.2005
dc.identifier.urihttps://hdl.handle.net/11468/23542
dc.identifier.volume73en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.relation.ispartofInfection and Immunity
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleUnusual genetic organization of a functional type I protein secretion system in Neisseria meningitidisen_US
dc.titleUnusual genetic organization of a functional type I protein secretion system in Neisseria meningitidis
dc.typeArticleen_US

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