A study for determining mechanism of subtilisin-casein interaction by using inhibition kinetics data

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dc.contributor.authorAytekin, C
dc.contributor.authorBaysal, ZK
dc.date.accessioned2024-04-24T17:33:12Z
dc.date.available2024-04-24T17:33:12Z
dc.date.issued1998
dc.departmentDicle Üniversitesien_US
dc.description.abstractCasein is a non-specific substrate for the an extacellular protease subtilisin. In this study tyrosine, tryptophan, cysteine, phenol and ethyl-methane sulfonate (EMS) were added to the enzyme-substrate medium in appropriate concentrations to investigate their kinetics effect. The additions of these compounds to incubation medium were found cause to different kinds of inhibitions. The inhibition effects of tyrosine, tryptophan and phenol indicate that the enzyme attacks the aromatic amino acid residues on the substrate. Because the sulfhydryl group of cysteine is a very strong induce protonates the imidazol groups of histidine on the enzyme. This can be explained as cysteine effected that the histidine residues decrease the nucleophilicity of the serine residues in the active site of enzyme. On the other hand the effect of mutagenic compound EMS caused to competitive inhibition. It was observed that this result to be in contradiction with alkylation reagents effect that cause to irreversible inhibition.en_US
dc.identifier.endpage58en_US
dc.identifier.issn0749-5331
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-0031919606
dc.identifier.scopusqualityN/A
dc.identifier.startpage49en_US
dc.identifier.urihttps://hdl.handle.net/11468/20523
dc.identifier.volume14en_US
dc.identifier.wosWOS:000072197700006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherMbr Press Incen_US
dc.relation.ispartofBiochemical Archives
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[No Keyword]en_US
dc.titleA study for determining mechanism of subtilisin-casein interaction by using inhibition kinetics dataen_US
dc.titleA study for determining mechanism of subtilisin-casein interaction by using inhibition kinetics data
dc.typeArticleen_US

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