Antioxidant, anticholinesterase activities and polyphenolic constituents of cones of algerian fir (Abies numidica) by LCESI- MS/MS with chemometric approach
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Tarih
2021
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Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
The fractions (Fr.1-5) of the hydromethanolic extract of Abies numidica cones were studied for theirpolyphenol constituents, antioxidant, and anticholinesterase activities. The ?-carotene-linoleic acid, cupric reducingpower (CUPRAC), DPPH scavenging, and ABTS radical scavenging assays were used to determine antioxidant activity.Fr.3 exhibited the highest antioxidant activity in ABTS test (IC50: 1.29 ?g/mL), b-carotene-linoleic acid test (IC50: 18.6?g/mL) and CUPRAC (A0.5: 33.8 ?g/mL) assays. Three fractions (Fr.2-4) promising antioxidant activity were analyzedusing LC-MS/MS for their phenolic compositions. Taxifolin (155.9-2816.2 ?g analyte/g extract), hyperoside (353.0-2045.5 ?g/g), vanillin (1488.9-1529.9 ?g/g), tannic acid (1281.6-1416.8 ?g/g), rosmarinic acid (1063.1-1149.3 ?g/g),coumarin (683.0-772.6 ?g/g), quercetin (881.0 ?g/g), and catechin (277.7-400.2 ?g/g) were identified in the antioxidantfractions. Moreover, the anticholinesterase activity was also performed using the in vitro spectroscopic Ellman method.The antioxidant fractions exhibited moderate butyrylcholinesterase inhibitory activity. The multi-ware analysis wasperformed to understand the origin of bioactivities. According to principal component analysis, it is detected that thehyperoside, catechin, taxifolin, and tannic acid were responsible for the antioxidant activity. Therefore, the cones ofAbies numidica can be considered as potent antioxidant and anticholinesterase sources in food and pharmaceuticalindustries, commercially.
Açıklama
Anahtar Kelimeler
Kaynak
Journal of research in pharmacy (online)
WoS Q Değeri
Scopus Q Değeri
Cilt
25
Sayı
3
