Qualitative and Quantitative Evaluation of Cytotoxicity of Five Different One-Step Self-Etching Adhesives

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dc.contributor.authorYildirim, Zehra Susgun
dc.contributor.authorBakir, Seyhmus
dc.contributor.authorBakir, Elif Pinar
dc.contributor.authorFoto, Egemen
dc.date.accessioned2024-04-24T17:20:45Z
dc.date.available2024-04-24T17:20:45Z
dc.date.issued2018
dc.departmentDicle Üniversitesien_US
dc.description.abstractPurpose: To qualitatively and quantitatively compare the cytotoxic potentials of five different one-step self-etching adhesives: Prime& Bond One-Select (PB-OS), Optibond All-in-One (OB-AIO), G-Bond (GB), Clearfil Universal Bond (CUB), Single Bond Universal (SBU). Materials and Methods: During the first stage of the study, the cytotoxic activities of the test materials were evaluated qualitatively using the direct contact method. In this method, the test materials were placed directly into a monkey kidney epithelial cell culture medium. Reaction zones which occurred in the culture medium were evaluated, in addition to the density and changes in the morphology of the cells. During the second stage, the cytotoxic potential of four different dilutions (1%, 0.1%, 0.01%, 0.001%) of the test materials on L929 rat fibroblast cells was quantitatively evaluated at three different time periods (24 h, 48 h, 72 h) with the MTT tetrazolium-based assay. Results: In the first stage, a zone exceeding 1 cm was observed around or below SBU, CUB, GB and OB-AIO. In PBOS, the zone borders were approximately 1 cm. In the second stage after the MTT assay, CUB was the most cytotoxic after 24 h, GB and SBU after 48 h, and OB-AIO after 72 h. Conclusion: All adhesives tested showed different degrees of cytotoxicity, which statistically significantly increased with dose. Changes were seen related to time.en_US
dc.description.sponsorshipDicle University, Turkey [DIS.15.026]en_US
dc.description.sponsorshipThe study was supported by the coordinator of scientific research projects at Dicle University, Turkey (Project No: DIS.15.026).en_US
dc.identifier.doi10.3290/j.ohpd.a41544
dc.identifier.endpage532en_US
dc.identifier.issn1602-1622
dc.identifier.issn1757-9996
dc.identifier.issue6en_US
dc.identifier.pmid30511051
dc.identifier.startpage525en_US
dc.identifier.urihttps://doi.org/10.3290/j.ohpd.a41544
dc.identifier.urihttps://hdl.handle.net/11468/19229
dc.identifier.volume16en_US
dc.identifier.wosWOS:000457800200007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherQuintessence Publishing Co Incen_US
dc.relation.ispartofOral Health & Preventive Dentistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCytotoxicityen_US
dc.subjectDirect Contact Methoden_US
dc.subjectMtten_US
dc.subjectOne-Step Adhesivesen_US
dc.subjectSelf-Etchingen_US
dc.titleQualitative and Quantitative Evaluation of Cytotoxicity of Five Different One-Step Self-Etching Adhesivesen_US
dc.titleQualitative and Quantitative Evaluation of Cytotoxicity of Five Different One-Step Self-Etching Adhesives
dc.typeArticleen_US

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