Identification and quantification of phenolic and volatile constituents in five different Anatolian thyme species using LC-MS/MS and GC-MS, with biological activities

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dc.contributor.authorBoga, Mehmet
dc.contributor.authorOzkan, Esra Eroglu
dc.contributor.authorErsoy, Ezgi
dc.contributor.authorTuncay, Eyyup
dc.contributor.authorCanturk, Yeter Yesil
dc.contributor.authorCinar, Ercan
dc.contributor.authorKara, Emel Mataraci
dc.date.accessioned2024-04-24T16:11:29Z
dc.date.available2024-04-24T16:11:29Z
dc.date.issued2021
dc.departmentDicle Üniversitesien_US
dc.description.abstractBeing widely used in the food industry, thyme is one of the most valuable plants in many aspects, and Turkey is one of the hotspots for thyme industry. Five thyme species, Thymus convolutus (endemic), Thymus fallax, Thymus haussknechtii Velen (endemic), Thymus kotschyanus var. kotschyanus, and Thymus sipyleus from Turkey were subjected in this study. Their essential oils and the extracts of aerial and root parts were obtained. GC-MS analyses showed that camphor was the major compound in T. convolutus (12.7%), and in T. sipyleus (13.1%) essential oils. The major volatile compound of T. fallax was bicyclogermacrene (21.5%), whereas the T. kotschyanus var. kotschyanus essential oil showed a predominance of carvacrol (48.5%), and thymol (22.5%). Carvacrol was also the major compound of T. haussknechtii with 28.2%. LC-MS/MS analyses showed that nicotiflorin (293.28-4201.15 mu g/mL), caffeic (516.41-851.28 mu g/mL), quinic (344.37-4410.94 mu g/mL), rosmarinic (1800.18-9631.71 mu g/mL), and fumaric acid (350.88-682.10 mu g/mL) were detected as the main compounds in the extracts. All extracts exerted strong antioxidant activity, and the highest radical scavenging activity was demonstrated by T. haussknechtii root extract. T. convolutus and T. haussknechtii aerial parts extracts showed significant antimicrobial activity against C. tropicalis at MIC of 19.53 mu g/mL. The extracts were found to be nontoxic against COLO205 and KM12 human colon, and UO-31, and A498 human renal cell lines. Their enzyme inhibitory activities were also evaluated against cholinesterase, tyrosinase and urease enzymes and T. fallax aerial part extract was the most effective with 34.48 +/- 0.60% in antibutyrylcholinesterase activity. These species with strong antioxidant and antifungal properties and no toxicity could be beneficial to be used as preservatives in the food processing industry.en_US
dc.description.sponsorshipDicle University Science and Technology Research and Application Center (DUBTAM) [D.U.BAP/14-EZF-69]; TUBITAK 2219-International Postdoctoral Research Fellowship Programen_US
dc.description.sponsorshipPart of this work is Eyyup Tuncay's master's thesis. LC-MS/MS analysis was performed by Dicle University Science and Technology Research and Application Center (DUBTAM), thanks to project number D.U.BAP/14-EZF-69 to pay the analysis fee. E. Eroglu Ozkan is also thankful to Dr. John A. Beutler, MTP (Molecular Targets Program), Assay Development and Screening Section, NCI (National Cancer Institute), Frederick, MD, USA and The Scientific and Technological Research Council of Turkey (TUBITAK), Ankara, Turkey. A part of the study is supported by TUBITAK 2219-International Postdoctoral Research Fellowship Program.en_US
dc.identifier.doi10.1016/j.fbio.2021.101141
dc.identifier.issn2212-4292
dc.identifier.issn2212-4306
dc.identifier.scopus2-s2.0-85107117094
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.fbio.2021.101141
dc.identifier.urihttps://hdl.handle.net/11468/15409
dc.identifier.volume43en_US
dc.identifier.wosWOS:000701894900004
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofFood Bioscience
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectThymus Speciesen_US
dc.subjectThymeen_US
dc.subjectFood Applicationsen_US
dc.subjectAntioxidanten_US
dc.subjectGc-Msen_US
dc.subjectLc-Ms/Msen_US
dc.titleIdentification and quantification of phenolic and volatile constituents in five different Anatolian thyme species using LC-MS/MS and GC-MS, with biological activitiesen_US
dc.titleIdentification and quantification of phenolic and volatile constituents in five different Anatolian thyme species using LC-MS/MS and GC-MS, with biological activities
dc.typeArticleen_US

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