Dicle Üniversitesi Tıp Fakültesi Hastanesi’nde bruselloz seroprevalansı: Rose Bengal Testi ve Brucella Coombs Test sonuçları
Tarih
2022
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dicle Üniversitesi Tıp Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Amaç: Bruselloz dünyanın birçok ülkesinde ve ülkemizde en yaygın görülen zoonotik hastalıklardan biridir. Bu
çalışmada; 2021 yılı içerisinde hastanemiz mikrobiyoloji laboratuvarına çeşitli klinik ve polikliniklerden bruselloz
şüphesi ile gönderilen hasta serum örneklerinde çalışılan RoseBengal Testi (RBT)ve BrucellaCoombs Testi (BCT)
aglütinasyon titrelerinin, bölgemizdeki bruselloz seroprevalansını belirlemek için retrospektif olarak irdelenmesi
amaçlanmıştır.
Yöntemler: Hastalardan serolojik testlerde kullanılmak üzere kan örnekleri alındıktan sonra serumları ayrıldı. Tarama
testi olarak RoseBengal lam aglütinasyon testi kullanılmıştır. Tarama testi pozitif bulunan hasta serumlarına, Brucella
immuncapture aglütinasyon testleri uygulanmıştır. Çalışmamızda uygulanan testlerde, BCT yöntemiyle 1/320 ve üzeri
bulunan titreler pozitif olarak değerlendirilmiştir.
Bulgular: Çalışmada değerlendirilen 5.196 bruselloz şüpheli hasta örneğinden; RBT pozitif olan 336 (%6,5) hasta
bulunmuştur. RBT pozitif olan serumların 257 tanesinin (%76,5) BCT titresi 1/320 ve üzerinde pozitif olarak
saptanmıştır. BCT seropozitifliği %62,2 oranında en sık 20-50 yaş grubunda görülmüştür. BCTseropozitifliğinin hastanın
başvurduğu kliniğe göre dağılımında ise en sık başvuru yapılan klinik %47,6 oranında enfeksiyon hastalıkları polikliniği
idi. BCT seropozitif serumların 176’sı (%52,4) kadın, 160’ı (%47,6) ise erkek hastalara aitti.
Sonuç: Bruselloz, fakültatif intrasellüler bakterilerle ortaya çıktığından birçok organ ve sistemi etkileyebilmektedir.
Hastalığın tanısında kültür altın standart olmakla birlikte serolojik testler de sıklıkla kullanılmaktadır. Bu çalışma ile elde
edilen retrospektif veriler doğrultusunda hastanemizde bruselloz seroprevalansı %5 olarak saptanmıştır. Bu çalışmada
serolojik test sonuçlarını değerlendirerek bölgesel verilerin oluşturulmasında katkı yarattığımızı düşünmekteyiz.
Objective: Brucellosis is one of the most common zoonotic diseases in many countries of the world and in our country. In this study; we aimed to analyze the Rose Bengal Test (RBT) and Brucella Coombs Test (BCT) agglutination titers retrospectively, which were studied in the serum samples of patients with suspected brucellosis sent to the microbiology laboratory of our hospital from various clinics and outpatient clinics in 2021, to determine the brucellosis seroprevalence in our region. Methods: After taking blood samples from the patients to be used in serological tests, their serums were separated. Rose Bengal slide agglutination test was used as screening test. Brucella immunocapture agglutination tests were applied to the sera of the patients with positive screening test. In the tests applied in our study, the titers found to be 1/320 and above by the BCT method were evaluated as positive. Results: Of the 5.196 brucellosis suspected patient samples evaluated in the study; There were 336 (6.5%) patients with positive RBT. The BCT titer of 257 (76.5%) of the serums with positive RBT was found to be positive at 1/320 and above. BCT seropositivity was seen most frequently in the 20-50 age group with a rate of 62.2%. In the distribution of BCT seropositivity according to the clinic that the patient applied to, the most frequently applied clinic was the infectious diseases outpatient clinic with a rate of 47.6%. Of the BCT seropositive sera; 176 (52.4%) belonged to female patients and 160 (47.6%) belonged to male patients. Conclusion: Brucellosis can affect many organs and systems since it occurs with facultative intracellular bacteria. Although culture is the gold standard in the diagnosis of the disease, serological tests are also frequently used. In line with the retrospective data obtained in this study, the brucellosis seroprevalence was found to be 5% in our hospital. In this study, we think that by evaluating the serological test results, we contributed to the creation of regional data.
Objective: Brucellosis is one of the most common zoonotic diseases in many countries of the world and in our country. In this study; we aimed to analyze the Rose Bengal Test (RBT) and Brucella Coombs Test (BCT) agglutination titers retrospectively, which were studied in the serum samples of patients with suspected brucellosis sent to the microbiology laboratory of our hospital from various clinics and outpatient clinics in 2021, to determine the brucellosis seroprevalence in our region. Methods: After taking blood samples from the patients to be used in serological tests, their serums were separated. Rose Bengal slide agglutination test was used as screening test. Brucella immunocapture agglutination tests were applied to the sera of the patients with positive screening test. In the tests applied in our study, the titers found to be 1/320 and above by the BCT method were evaluated as positive. Results: Of the 5.196 brucellosis suspected patient samples evaluated in the study; There were 336 (6.5%) patients with positive RBT. The BCT titer of 257 (76.5%) of the serums with positive RBT was found to be positive at 1/320 and above. BCT seropositivity was seen most frequently in the 20-50 age group with a rate of 62.2%. In the distribution of BCT seropositivity according to the clinic that the patient applied to, the most frequently applied clinic was the infectious diseases outpatient clinic with a rate of 47.6%. Of the BCT seropositive sera; 176 (52.4%) belonged to female patients and 160 (47.6%) belonged to male patients. Conclusion: Brucellosis can affect many organs and systems since it occurs with facultative intracellular bacteria. Although culture is the gold standard in the diagnosis of the disease, serological tests are also frequently used. In line with the retrospective data obtained in this study, the brucellosis seroprevalence was found to be 5% in our hospital. In this study, we think that by evaluating the serological test results, we contributed to the creation of regional data.
Açıklama
Anahtar Kelimeler
Bruselloz, Seroprevalans, Tanı, Brucellosis, Seroprevalence, Diagnosis
Kaynak
Dicle Tıp Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
49
Sayı
4
Künye
Temiz, H., Özcan, N., Araç, E. ve Özbek, E. (2022). Dicle Üniversitesi Tıp Fakültesi Hastanesi’nde bruselloz seroprevalansı: Rose Bengal Testi ve Brucella Coombs Test sonuçları. Dicle Tıp Dergisi, 49(4), 660-666.
