ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF THERMOSTABLE XYLANASE FROM THERMOPHILIC ANOXYBACILLUS SP ISOLATED FROM HOT SPRINGS

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dc.contributor.authorEnez, Baris
dc.contributor.authorFincan, Sema Aguloglu
dc.date.accessioned2024-04-24T17:40:04Z
dc.date.available2024-04-24T17:40:04Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractIsolation, partial purification and characterization of thermostable xylanase from thermophilic Anoxybacillus isolated from hot springs located in Afyonkarahisar were performed. The bacterium was determined to be bacilli (rods) shape, gram-positive and movable. Optimum conditions for bacterial growth and enzyme production were determined to be 55 degrees C, pH 7.0 and 48 hours. Maximum anzyme activity was reached at 60 degrees C and pH 7.0. Partial purification of xylanase enzyme was performed with precipitation of 80% ammonium sulfate and dialysis procedures (1.2-fold and 35% recovery, respectively). The molecular weight of xylanase was similar to 34 kDa, as estimated by SDS-PAGE. Partially purified anzyme was observed to maintain its stability after 2 hours in 50 degrees C and 60 degrees C and after 1 hour in 70 degrees C temperature. It was determined that enzyme activity was maintained 100% at the end of the 1-hour period at pH 6.0, 7.0 and 8.0. A particularly strong inhibition was observed with the SDS (83%) effect caused by detergents used on enzyme inhibition.en_US
dc.description.sponsorshipABCen_US
dc.description.sponsorshipThe authors thank the financial supports from the ABC. The authors gratefully acknowledge the XYZ Institute of Applied Physics which provided beam time and facilities for ABC analysis.en_US
dc.identifier.endpage3048en_US
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue8en_US
dc.identifier.startpage3038en_US
dc.identifier.urihttps://hdl.handle.net/11468/21583
dc.identifier.volume25en_US
dc.identifier.wosWOS:000384033100040
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherParlar Scientific Publications (P S P)en_US
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnoxybacillus Sp.en_US
dc.subjectXylanaseen_US
dc.subjectPartial Purificationen_US
dc.subjectIsolationen_US
dc.titleISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF THERMOSTABLE XYLANASE FROM THERMOPHILIC ANOXYBACILLUS SP ISOLATED FROM HOT SPRINGSen_US
dc.titleISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF THERMOSTABLE XYLANASE FROM THERMOPHILIC ANOXYBACILLUS SP ISOLATED FROM HOT SPRINGS
dc.typeArticleen_US

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