ISOLATION, PARTIAL PURIFICATION AND CHARACTERIZATION OF THERMOSTABLE XYLANASE FROM THERMOPHILIC ANOXYBACILLUS SP ISOLATED FROM HOT SPRINGS
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Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Isolation, partial purification and characterization of thermostable xylanase from thermophilic Anoxybacillus isolated from hot springs located in Afyonkarahisar were performed. The bacterium was determined to be bacilli (rods) shape, gram-positive and movable. Optimum conditions for bacterial growth and enzyme production were determined to be 55 degrees C, pH 7.0 and 48 hours. Maximum anzyme activity was reached at 60 degrees C and pH 7.0. Partial purification of xylanase enzyme was performed with precipitation of 80% ammonium sulfate and dialysis procedures (1.2-fold and 35% recovery, respectively). The molecular weight of xylanase was similar to 34 kDa, as estimated by SDS-PAGE. Partially purified anzyme was observed to maintain its stability after 2 hours in 50 degrees C and 60 degrees C and after 1 hour in 70 degrees C temperature. It was determined that enzyme activity was maintained 100% at the end of the 1-hour period at pH 6.0, 7.0 and 8.0. A particularly strong inhibition was observed with the SDS (83%) effect caused by detergents used on enzyme inhibition.
Açıklama
Anahtar Kelimeler
Anoxybacillus Sp., Xylanase, Partial Purification, Isolation
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
25
Sayı
8
