Purification and covalent immobilization of benzaldehyde lyase with heterofunctional chelate-epoxy modified magnetic nanoparticles and its carboligation reactivity

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dc.contributor.authorTural, Bilsen
dc.contributor.authorTural, Servet
dc.contributor.authorErtas, Erdal
dc.contributor.authorYalinkilic, Ibrahim
dc.contributor.authorDemir, Ayhan S.
dc.date.accessioned2024-04-24T16:15:32Z
dc.date.available2024-04-24T16:15:32Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn this work, histidine-tagged recombinant benzaldehyde lyase from Pseudomonas fluorescens Biovar I (BAL, EC 4.1.2.38) was immobilized on the magnetically responsive epoxy-chelate magnetic support following a two-step mechanism; that is, the protein is physically adsorbed and then the covalent bonding takes place. This mechanism has been exploited to combine the selectivity of metal chelate affinity chromatography with the covalent immobilization capacity of epoxy supports. In this way, it has been possible to accomplish, in a simple manner, the purification, immobilization, and stabilization of a histidine-tagged recombinant benzaldehyde lyase. To fulfill this objective we prepared and characterized a multifunctional Co2+-IDA-epoxy functionalized the silica coated magnetic nanoparticles (SCMPs) which are modified with glycidyloxypropyltrimethoxysilane (GPTMS) and iminodiacetic acid (IDA). To test immobilized BAL, benzoin condensation reaction was performed with this magnetically responsive biocatalyst. The results obtained from the carboligation reaction that was performed with this simple and convenient heterogeneous biocatalyst were comparable to that of free-enzyme-catalyzed reaction. Additional advantages are its reusability and it is easy to work with. (c) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipMiddle East Technical University; Dicle University Research Fund (DUBAP) [13-ZEF-28, 10-ZEF-129, 10-ZEF-28, 10-ZEF-128, 12-ZEF-25]; Scientific and Technological Research Council of Turkey (TUBITAK) [109T497]; COST Action [CM0701]en_US
dc.description.sponsorshipThis project is funded by the financial support from Middle East Technical University, Dicle University Research Fund (DUBAP, Project No. 13-ZEF-28, 10-ZEF-129, 10-ZEF-28 and 10-ZEF-128, 12-ZEF-25) and the Scientific and Technological Research Council of Turkey (TUBITAK, 109T497), COST Action CM0701.en_US
dc.identifier.doi10.1016/j.molcatb.2013.05.023
dc.identifier.endpage47en_US
dc.identifier.issn1381-1177
dc.identifier.issn1873-3158
dc.identifier.scopus2-s2.0-84879227606
dc.identifier.scopusqualityN/A
dc.identifier.startpage41en_US
dc.identifier.urihttps://doi.org/10.1016/j.molcatb.2013.05.023
dc.identifier.urihttps://hdl.handle.net/11468/15842
dc.identifier.volume95en_US
dc.identifier.wosWOS:000322612100007
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal of Molecular Catalysis B-Enzymatic
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectImmobilized Benzaldehyde Lyaseen_US
dc.subjectEpoxy-Chelate Magnetic Supporten_US
dc.subjectMetal-Affinity Magnetic Nanoparticlesen_US
dc.subjectSelective Immobilizationen_US
dc.subjectMultipoint Covalent Attachmenten_US
dc.subjectOne Step Purificationen_US
dc.titlePurification and covalent immobilization of benzaldehyde lyase with heterofunctional chelate-epoxy modified magnetic nanoparticles and its carboligation reactivityen_US
dc.titlePurification and covalent immobilization of benzaldehyde lyase with heterofunctional chelate-epoxy modified magnetic nanoparticles and its carboligation reactivity
dc.typeArticleen_US

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