Purification and characterization of novel ?-amylase from Anoxybacillus ayderensis FMB1

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dc.contributor.authorBekler, Fatma Matpan
dc.contributor.authorGuven, Kemal
dc.contributor.authorGuven, Reyhan Gul
dc.date.accessioned2024-04-24T16:24:48Z
dc.date.available2024-04-24T16:24:48Z
dc.date.issued2021
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe thermophilic amylase producing Anoxybacillus ayderensis FMB1 was isolated from Sorgun Hot Spring in Turkey. In order to obtain improved production of the enzyme for cost-effective process, optimization of the process parameters is required. Optimum conditions for amylase production were determined using the traditional Submerged Fermentation (SmF) method. The maximum amylase activity was 295.8 U/mg, under the optimized conditions (24-h, 55 degrees C, and pH 8.0) in liquid BMB medium. The best enzyme production was obtained in the presence of 1.5% soluble starch as carbon source and 1% yeast extract as nitrogen source. Enzyme was produced under optimal conditions and partially purified. Partially purified enzyme was stable for up to 120 minutes at 50 degrees C, and its stability was found to be pH 5.0-10.0. Regarding the effects of some metals and chemicals on the activity of the partially purified enzyme, Ca2+ increased the activity by 21%, but enzyme activity was decreased by Mg2+, Zn2+, Ni2+, and Cu2+ by 5%, 29%, 57%, and 64%, respectively. and the enzyme activity was completely inhibited by Hg2+. In addition; DTT, PMSF, PCMB, SDS and EDTA caused a decrease in enzyme activity by 3%, 4%, 12%, 27% and 77%, respectively. The molecular weight of enzyme was found to be 58.5 kDa by SDS-PAGE. The results obtained show that the thermophilic A. ayderensis FMB1 and their enzyme have biotechnological importance.en_US
dc.identifier.doi10.1080/10242422.2020.1856097
dc.identifier.endpage332en_US
dc.identifier.issn1024-2422
dc.identifier.issn1029-2446
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85097150982
dc.identifier.scopusqualityQ2
dc.identifier.startpage322en_US
dc.identifier.urihttps://doi.org/10.1080/10242422.2020.1856097
dc.identifier.urihttps://hdl.handle.net/11468/16860
dc.identifier.volume39en_US
dc.identifier.wosWOS:000596246300001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofBiocatalysis and Biotransformation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmylaseen_US
dc.subjectCa-Dependenten_US
dc.subjectThermostableen_US
dc.titlePurification and characterization of novel ?-amylase from Anoxybacillus ayderensis FMB1en_US
dc.titlePurification and characterization of novel ?-amylase from Anoxybacillus ayderensis FMB1
dc.typeArticleen_US

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