Effects of radio-frequency fields on bacterial cell membranes and nematode temperature-sensitive mutants

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dc.contributor.authorGul Guven, Reyhan
dc.contributor.authorGuven, Kemal
dc.contributor.authorDawe, Adam
dc.contributor.authorWorthington, John
dc.contributor.authorHarvell, Christopher
dc.contributor.authorPopple, Amy
dc.contributor.authorSmith, Tim
dc.date.accessioned2024-04-24T16:11:24Z
dc.date.available2024-04-24T16:11:24Z
dc.date.issued2006
dc.departmentDicle Üniversitesien_US
dc.description.abstractMembrane-related bioeffects have been reported in response to both radio-frequency (RF) and extremely low-frequency (ELF) electromagnetic fields (EMFs), particularly in neural cells. We have tested whether RF fields might cause inner membrane leakage in ML35 E. coli cells, which express beta-galactosidase (lacZ) constitutively, but lack the lacY permease required for substrate entry. The activity of lacZ (indicating substrate leakage through the inner cell membrane) was increased only slightly by RF exposure (1 GHz, 0.5 W) over 45 min. Since lacZ activity showed no further increase with a longer exposure time of 90 min, this suggests that membrane permeability per se is not significantly affected by RF fields, and that slight heating (<= 0.2 degrees C) could account for this small difference. Temperature-sensitive (ts) mutants of the nematode, Caenorhabditis elegans, are wild-type at 15 degrees C but develop the mutant phenotype at 25 degrees C; an intermediate temperature of 21 degrees C results in a reproducible mixture of both phenotypes. For two ts mutants affecting transmembrane receptors (TRA-2 and GLP-1), RF exposure for 24 h during the thermocritical phase strongly shifts the phenotype mix at 21 degrees C towards the mutant end of the spectrum. For ts mutants affecting nuclear proteins, such phenotype shifts appear smaller (PHA-1) or non-significant (LIN-39), apparently confirming suggestions that RF power is dissipated mainly in the plasma membrane of cells. However, these phenotype shifts are no longer seen when microwave treatment is applied at 21 degrees C in a modified exposure apparatus that minimises the temperature difference between sham and exposed conditions. Like other biological effects attributed to microwaves in the C. elegans system, phenotype shifts in ts mutants appear to be an artefact caused by very slight heating. (c) 2006 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.enzmictec.2005.12.017
dc.identifier.endpage795en_US
dc.identifier.issn0141-0229
dc.identifier.issn1879-0909
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-33745201225
dc.identifier.scopusqualityQ1
dc.identifier.startpage788en_US
dc.identifier.urihttps://doi.org/10.1016/j.enzmictec.2005.12.017
dc.identifier.urihttps://hdl.handle.net/11468/15377
dc.identifier.volume39en_US
dc.identifier.wosWOS:000238932500040
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofEnzyme and Microbial Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicrowavesen_US
dc.subjectElectromagnetic Fieldsen_US
dc.subjectBacterial Inner Membraneen_US
dc.subjectCaenorhabditis Elegansen_US
dc.subjectTemperature-Sensitive Mutantsen_US
dc.titleEffects of radio-frequency fields on bacterial cell membranes and nematode temperature-sensitive mutantsen_US
dc.titleEffects of radio-frequency fields on bacterial cell membranes and nematode temperature-sensitive mutants
dc.typeArticleen_US

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