Determination of enzyme inhibition potential and anticancer effects of pistacia khinjuk stocks raised in in vitro and in vivo conditions

Basit Öğe Kaydı
dc.authorid0000-0002-0988-9462en_US
dc.authorid0000-0002-2193-8386en_US
dc.contributor.authorTilkat, Emine Ayaz
dc.contributor.authorBatıbay, Hayri
dc.contributor.authorYener, İsmail
dc.contributor.authorYılmaz, Pelin Köseoğlu
dc.contributor.authorAkdeniz, Mehmet
dc.contributor.authorKaplan, Alevcan
dc.contributor.authorErcişli, Sezai
dc.contributor.authorErtas, Abdulselam
dc.contributor.authorHolubec, Vojtech
dc.date.accessioned2021-04-14T11:04:13Z
dc.date.available2021-04-14T11:04:13Z
dc.date.issued2021en_US
dc.departmentDicle Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri Bölümüen_US
dc.descriptionWOS:000609672100001
dc.description.abstractIn this study, antihypertensive, anticholinesterase, antiurease, antityrosinase and antielastase enzyme inhibition and anticancer activities of in vivo (male and female) and in vitro samples (root, stem and leaf parts) of the Pistacia khinjuk Stocks were investigated comparatively. In this context, in vitro shoot cultures were obtained from germinated mature seeds. Then, the juvenile shoots were proliferated in Murashige and Skoog (MS) medium supplemented with 1 mg/L 6-Benzylaminopurine (BAP). In terms of anticancer activity, the whole of the samples studied was found to have apoptotic effects against MCF-7 (breast cancer) and HT-29 (colon cancer) cell lines. The extracts obtained from in vivo female root parts showed better cytotoxicity than all the other tested extracts on MCF-7 (IC50: 31.86 +/- 1.40 mu g/mL) and HT-29 cell series (IC50: 59.60 +/- 0.69 mu g/mL). Even though all the samples showed a strong butyrylcholinesterase enzyme inhibition (BChE) activity, it was detected that none of the samples had shown acetylcholinesterase enzyme inhibition (AChE). It was also determined that in vivo leaf samples of female trees had the highest BChE activity (Inhibition%: 75.20 +/- 1.50). All the samples showed a low-moderate level of urease and tyrosinase enzyme activity, while in vivo samples showed a significant level of the elastase enzyme activities (Inhibition%: 58.72 for female root extracts; 58.25 for female leaf extracts, at 50 mu g/mL concentration), and they were more active than the standard oleanolic acid (Inhibition%: 39.46 +/- 0.52). The antihypertensive activities as the inhibition of angiotensin I-converting enzyme (ACE) of in vivo samples (Inhibition%: 95.88 for female stem extracts; 95.18 for female root extracts) were detected as close to the standard (Inhibition%: 96.64 +/- 1.85) used. In general, it can be stated that in vivo samples had higher biological activities compared to in vitro ones. Consequently, according to our results, it was concluded that in vitro stem parts of khinjuk pistachio could also be evaluated as an alternative new antihypertensive, antielastase and anticancer agent source.en_US
dc.identifier.citationTilkat, E.A., Batıbay, H., Yener, İ., Yılmaz, P.K., Akdeniz, M., Kaplan, A. ve diğerleri. (2021). Determination of enzyme inhibition potential and anticancer effects of pistacia khinjuk stocks raised in in vitro and in vivo conditions. Agronomy, 11(1).en_US
dc.identifier.doi10.3390/agronomy11010154
dc.identifier.issn2073-4395
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85109005671
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://www.mdpi.com/2073-4395/11/1/154
dc.identifier.urihttps://hdl.handle.net/11468/6706
dc.identifier.volume11en_US
dc.identifier.wosWOS:000609672100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorYener, İsmail
dc.institutionauthorErtas, Abdulselam
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.relation.ispartofAgronomy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPistacia khinjuken_US
dc.subjectin vitro and in vivoen_US
dc.subjectCytotoxic activityen_US
dc.subjectAntihypertensiveen_US
dc.subjectAnticholinesteraseen_US
dc.subjectAntiureaseen_US
dc.subjectAntityrosinaseen_US
dc.subjectAntielastase activityen_US
dc.titleDetermination of enzyme inhibition potential and anticancer effects of pistacia khinjuk stocks raised in in vitro and in vivo conditionsen_US
dc.titleDetermination of enzyme inhibition potential and anticancer effects of pistacia khinjuk stocks raised in in vitro and in vivo conditions
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Determination of Enzyme Inhibition Potential and Anticancer.pdf
Boyut:
1.5 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Makale Dosyası
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Eczacılık Fakültesi Eczacılık Teknolojisi Bölümü Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu