Indirect somatic embryogenesis from mature embryo cultures of pistachio, Pistacia vera L.

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dc.contributor.authorOnay, Ahmet
dc.contributor.authorTilkat, Engin
dc.contributor.authorYildirim, Hakan
dc.contributor.authorSuzerer, Veysel
dc.date.accessioned2024-04-24T17:37:32Z
dc.date.available2024-04-24T17:37:32Z
dc.date.issued2007
dc.departmentDicle Üniversitesien_US
dc.description.abstractInduction of somatic embryogenesis, proliferation and development of somatic embryos was obtained through different culture passages with respect to plant growth regulators. Callus cultures were initiated on callus induction medium (CIM) containing Murashige and Skoog (MS) salts with Gamborg vitamins supplemented with 2.0 mg l(-1)2,4-dichlorophenoxyacetic acid (2,4-D) and 40 g l(-1) sucrose from mature zygotic embryo of pistachio. Embryogenic tissue with globular somatic embryos was obtained when the calli were initiated on Murashige and Skoog (MS) medium with Gamborg vitamins supplemented with 1-4 mg l(-1) 2,4-D or its combination with 6-benzylaminopurine (BAP). The embryogenic cultures were maintained on an embryogenesis induction medium (EIM), which contains 1-2 mg l(-1) BAP for 4 weeks. These cultures were regularly subcultured every three or four weeks on EIM. After transfer of the embryogenic tissues into the somatic embryo maturation medium (ENM) containing 0.5 mg l(-1) BAP, with various concentrations of abscisic acid (ABA) and sucrose, somatic embryos appeared. On the media with 0.5 mg P ABA and 6% sucrose the highest number of somatic embryos (42 per 250 mg of fresh weight) was formed. Adventive stages of somatic embryos were manually separated from the friable embryogenic tissues. Separated somatic embryos germinated on solidified MS medium without growth regulators, developed into plantlets.en_US
dc.identifier.endpage74en_US
dc.identifier.issn1311-9109
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-34547436711
dc.identifier.scopusqualityQ4
dc.identifier.startpage68en_US
dc.identifier.urihttps://hdl.handle.net/11468/21004
dc.identifier.volume7en_US
dc.identifier.wosWOS:000247326900004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSejani Publisheren_US
dc.relation.ispartofPropagation of Ornamental Plants
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPistacia Vera L.en_US
dc.subjectSomatic Embryogenesisen_US
dc.subjectZygotic Embryosen_US
dc.titleIndirect somatic embryogenesis from mature embryo cultures of pistachio, Pistacia vera L.en_US
dc.titleIndirect somatic embryogenesis from mature embryo cultures of pistachio, Pistacia vera L.
dc.typeArticleen_US

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