Investigation of Functional Sperm Membrane Integrity After Consequence of Anti-Hypoosmotic Swelling Test Technique An Electron Microscopic Study

Basit Öğe Kaydı
dc.contributor.authorTutsi, Yekbun
dc.contributor.authorAkkus, Murat
dc.contributor.authorTunik, Selcuk
dc.contributor.authorNergiz, Yusuf
dc.contributor.authorKaplanoglu, Iskender
dc.contributor.authorNasir, Yasemin
dc.date.accessioned2024-04-24T17:47:32Z
dc.date.available2024-04-24T17:47:32Z
dc.date.issued2015
dc.departmentDicle Üniversitesien_US
dc.description.abstractOBJECTIVE: Infertility is described as the inability of a couple to conceive after 1 year of unprotected intercourse. Male factor is assumed to be responsible for 50% of cases of infertile couples. It is a common clinical problem, affecting approximately 13 15% of couples worldwide.. In this study we aimed to investigate the effects of hypoosmotic swelling (HOS) and anti-HOS tests on sperm membrane integrity at the ultrastructural level. STUDY DESIGN: Twenty-nine infertile and 10 fertile men were included in this study. The fertile and infertile subjects were classified according to Kruger and WHO criterion. All semen samples were examined and analyzed based on WHO guidelines. Sperm viability was determined by using the eosin Y staining method. After HOS and anti-HOS tests were applied, the samples were evaluated at the ultrastructural level. RESULTS: Normal structural features of all regions of sperm were observed in sections of sham normospermia. Some histopathological changes were seen in HOS and anti-HOS group sections. CONCLUSION: The HOS procedure was found not to cause degenerative changes in the, sperm ultrastructure. The anti-HOS procedure can be applied in normospermic and oligospermic groups.en_US
dc.description.sponsorshipScientific Research Fund of University of Dicleen_US
dc.description.sponsorshipSupported by the Scientific Research Fund of University of Dicle.en_US
dc.identifier.endpage148en_US
dc.identifier.issn0024-7758
dc.identifier.issn1943-3565
dc.identifier.issue3-4en_US
dc.identifier.pmid25898477
dc.identifier.startpage141en_US
dc.identifier.urihttps://hdl.handle.net/11468/22583
dc.identifier.volume60en_US
dc.identifier.wosWOS:000351968600008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSci Printers & Publ Incen_US
dc.relation.ispartofJournal of Reproductive Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnti-Hypoosmotic Swelling Testen_US
dc.subjectHypoosmotic Swellingen_US
dc.subjectInfertilityen_US
dc.subjectIntracytoplasmic Sperm Injectionen_US
dc.subjectOligospermiaen_US
dc.subjectOligozoospermiaen_US
dc.subjectSpermen_US
dc.subjectSpermatozoaen_US
dc.subjectUltrastructuresen_US
dc.titleInvestigation of Functional Sperm Membrane Integrity After Consequence of Anti-Hypoosmotic Swelling Test Technique An Electron Microscopic Studyen_US
dc.titleInvestigation of Functional Sperm Membrane Integrity After Consequence of Anti-Hypoosmotic Swelling Test Technique An Electron Microscopic Study
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu