6-phosphogluconate dehydrogenase: Purification, characterization and kinetic properties from rat erythrocytes

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dc.contributor.authorBeydemir, O
dc.contributor.authorÇiftçi, M
dc.contributor.authorYilmaz, H
dc.contributor.authorKüfrevioglü, OI
dc.date.accessioned2024-04-24T17:33:10Z
dc.date.available2024-04-24T17:33:10Z
dc.date.issued2004
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn this paper, a simple and rapid method for the purification of 6-phosphogluconate dehydrogenase from rat erythrocytes together with an analysis of the kinetic behavior and some properties of the enzyme are considered. The purification steps comprised high-speed centrifugation, 20-50% ammonium sulfate precipitation and 2', 5'-ADP Sepharose 4B affinity gel chromatography. The yield was 78.4% and the specific enzyme activity was 5.15 EU/mg proteins. The molecular mass of the subunit was estimated to be 59,566 Da by SIDS polyacrylamide gel electrophoresis (SDS-PAGE) and native enzyme was found to be 111,000 Da by gel filtration column chromatography. The enzyme had an optimal pH at 7.0 and stable pH at 8.0 in 1 M Tris-HCI buffer, and optimal temperature at 45 degreesC. K-M and V-MAX for NADP(+) and 6-PGA as substrates were also determined. The inhibitor effects of ATP, NADPH and NADH were also examined, and K, values and the types of inhibition were determined by means of a Lineweaver-Burk graph obtained for them.en_US
dc.identifier.endpage714en_US
dc.identifier.issn1300-0128
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-7044233201
dc.identifier.scopusqualityQ3
dc.identifier.startpage707en_US
dc.identifier.urihttps://hdl.handle.net/11468/20486
dc.identifier.volume28en_US
dc.identifier.wosWOS:000224310000012
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject6pgden_US
dc.subjectRaten_US
dc.subjectErythrocyteen_US
dc.subjectPurificationen_US
dc.subjectKinetic Propertiesen_US
dc.title6-phosphogluconate dehydrogenase: Purification, characterization and kinetic properties from rat erythrocytesen_US
dc.title6-phosphogluconate dehydrogenase: Purification, characterization and kinetic properties from rat erythrocytes
dc.typeArticleen_US

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