Investigation of the Effects of Carvacrol on Experimental Ischemia/Reperfusion Model of Rat Ovaries by Immunohistochemistry

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dc.contributor.authorPeker, Nurullah
dc.contributor.authorDeger, Ugur
dc.contributor.authorAsir, Firat
dc.date.accessioned2024-04-24T17:37:34Z
dc.date.available2024-04-24T17:37:34Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractOBJECTIVE: Ovarian torsion is a gynecologic problem that presents as acute lower abdominal pain. As a result, blood flow to the ovaries decreases and ischemia develops. Many medications have been used to treat ovarian torsion, including carvacrol. We conducted an experimental ischemia/reperfusion (torsion-detorsion) model using rats to observe the effects of carvacrol on ovarian torsion by immunohistochemical study. STUDY DESIGN: Thirty-two female Wistar rats were randomly categorized into 4 groups (8 rats per group): control group, ischemia group, ischemia/reperfusion group, and ischemia/reperfusion + carvacrol-treated group. Ischemia was created by sealing the left ovaries for 3 hours, followed by 3-hour reperfusion. For the ischemia/reperfusion + carvacrol-treated group, 100 mg/kg carvacrol was administered orally in 2 mL 0.9% NaCl after the reperfusion. All animals were sacrificed, and ovarian tissues were dissected for routine paraffin wax embedding tissue protocol. RESULTS: Control groups showed normal ovarian histological structures. In the ischemia group, hyperplastic granulosa cell vascular dilation, severe hemorrhage, and inflammation were observed. The ischemia/reperfusion group showed edema, inflammation, congestion, degenerated follicles, and cells with pyknotic nuclei. In the ischemia/reperfusion + carvacrol group, degenerative changes and vascular pathologies were decreased in ovarian tissues. Endothelin-1 (ET-1) was expressed in degenerated follicles and vascular endothelial cells in the ischemia and ischemia/reperfusion group. Expression of ET-1 was decreased in follicular cells but negative in stromal and luteal cells in the ischemia/reperfusion + carvacrol group. ADAMTS-5 expression was positive in degenerated follicles, apoptotic cells, and inflammatory cells in the ischemia and ischemia/reperfusion groups. In the ischemia/reperfusion + carvacrol group, corona cells and a few inflammatory cells around vessels showed positive ADAMTS-5 expression. CONCLUSION: ET-1 can induce angiogenic development with the decrease of degenerative development and inflammation in the carvacrol group. ADAMTS-5 molecule may be a marker of cellular structure and extracellular matrix development in different stages of development of ovarian cells and follicles in ischemia and oxygen deficiency caused by ischemia reperfusion.en_US
dc.identifier.endpage204en_US
dc.identifier.issn0884-6812
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85119491437
dc.identifier.scopusqualityQ4
dc.identifier.startpage197en_US
dc.identifier.urihttps://hdl.handle.net/11468/21039
dc.identifier.volume42en_US
dc.identifier.wosWOS:000619650600004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSci Printers & Publ Incen_US
dc.relation.ispartofAnalytical and Quantitative Cytopathology and Histopathology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAdamts-5en_US
dc.subjectCarvacrolen_US
dc.subjectDetorsionen_US
dc.subjectEndothelin-1en_US
dc.subjectIschemiaen_US
dc.subjectIschemia-Reperfusion Injuryen_US
dc.subjectOvarian Torsionen_US
dc.subjectRatsen_US
dc.subjectWistaren_US
dc.subjectReperfusionen_US
dc.subjectReperfusion Injuryen_US
dc.subjectTorsion Abnormalityen_US
dc.titleInvestigation of the Effects of Carvacrol on Experimental Ischemia/Reperfusion Model of Rat Ovaries by Immunohistochemistryen_US
dc.titleInvestigation of the Effects of Carvacrol on Experimental Ischemia/Reperfusion Model of Rat Ovaries by Immunohistochemistry
dc.typeArticleen_US

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