Effect of different transport temperatures of cattle and sheep ovaries on in vitro maturation of oocytes

Basit Öğe Kaydı
dc.contributor.authorÖzdas, OB
dc.contributor.authorTas, M
dc.contributor.authorUmut, C
dc.contributor.authorMithat, E
dc.contributor.authorBacinoglu, S
dc.contributor.authorKamber, D
dc.contributor.authorAk, K
dc.date.accessioned2024-04-24T17:33:33Z
dc.date.available2024-04-24T17:33:33Z
dc.date.issued2006
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim of study is to determine the effects of different transport temperatures (4 degrees C, 32 degrees C) of sheep and cattle ovaries on the in vitro maturation of oocytes. Two experimental groups were formed. Sheep and cattle ovaries were put into saline solution at 32 degrees C. The ovaries were transported to the laboratory, at the same temperature (Group I) or at 4 degrees C following 10 minutes of incubation at room temperature (Group II) (n=6). Oocytes were collected from ovaries using the dissection method. Oocytes matured in their own group in 700 ml TCM-199 (supplemented with pyruvate, LH, FCS) for 23 h at a gas atmosphere of 5% CO2, 5% O2, 90% N2 and at 38.8 degrees C. After maturation, oocytes were fixed in acetic acid-ethyl alcohol (1:3) for 48 hours. The stages of development up to MII, of the oocytes stained with aceto-orsein were then examined. The Chi-Square test was used for statistical analysis. While in the VC group, sheep oocytes reached 30.6% (MI), 15.3% (MII) and cattle oocytes reached 17.3% (MI), 46.8% (MII), in the 32 degrees C group these percentages were respectively 38.3%, 33.1% in sheep and 19.3%, 55.4% in cattle. While oocytes obtained from sheep ovaries transported at 32 degrees C reached the MIII stage at a higher rate compared to those at 40C (P < 0.001), no statistically significant difference was observed between maturation to the MII stage of oocytes obtained from cattle ovaries transported at 4 degrees C and 32 degrees C. As a result of this study, it was established that cattle ovaries could be transported both at +4 C, +32 degrees C and that there was no difference in oocyte maturation.en_US
dc.identifier.endpage164en_US
dc.identifier.issn0025-8628
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-33644521565
dc.identifier.scopusqualityQ4
dc.identifier.startpage162en_US
dc.identifier.urihttps://hdl.handle.net/11468/20743
dc.identifier.volume62en_US
dc.identifier.wosWOS:000235023300011
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoplen_US
dc.publisherPolish Soc Veterinary Sciences Editorial Officeen_US
dc.relation.ispartofMedycyna Weterynaryjna-Veterinary Medicine-Science and Practice
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectOvary Transporten_US
dc.subjectIn Vitro Maturationen_US
dc.subjectBovineen_US
dc.subjectSheepen_US
dc.titleEffect of different transport temperatures of cattle and sheep ovaries on in vitro maturation of oocytesen_US
dc.titleEffect of different transport temperatures of cattle and sheep ovaries on in vitro maturation of oocytes
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu