Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers

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dc.contributor.authorKilinc, Fatih Mehmet
dc.contributor.authorSuzerer, Veysel
dc.contributor.authorCiftci, Yelda Ozden
dc.contributor.authorOnay, Ahmet
dc.contributor.authorYildirim, Hakan
dc.contributor.authorUncuoglu, Ahu Altinkut
dc.contributor.authorTilkat, Engin
dc.date.accessioned2024-04-24T16:02:10Z
dc.date.available2024-04-24T16:02:10Z
dc.date.issued2015
dc.departmentDicle Üniversitesien_US
dc.description.abstractAn efficient protocol for clonal micropropagation of selected genotypes of lentisk, Pistacia lentiscus L., which is cultivated for the masticha resin, has been developed using shoot tip explants originating from in vitro seedlings. BA was found to be optimum for shoot morphogenesis in terms of the number and length of shoots among the cytokinins tested for all cloned genotypes, while the highest shoot length was noticed in the presence of 2iP at a rate 4.92 mu M. Efficient rooting (94.15 %) was achieved in a medium containing 19.6 mu M IBA with the clone II that was superior to the rest of the clones tested. The method developed for plant acclimatization was satisfactory because a high percentage of plant survival (95 %) in the growth room in the clone II was obtained and the regenerated plantlets resumed growth after 4 months. DNAs from mother seedlings and micropropagated plantlets belonging to 6, 9 and 12 times subcultured were isolated and subjected to IRAP analysis in order to evaluate their genetic stability and detect possibly existing variations among in vitro derived plantlets. The mean percentage of similarity calculated by Jaccard's similarity coefficient ranged from 78 to 86 % in the four genotypes. Although variation was observed among mother plantlets and its regenerants for all of the clones, polymorphic information content value in the range of 0.391-0.405 indicated the presence of reasonable polymorphism within the clones. The presented data confirmed that the clonal propagation of lentisk by using shoot tips could be used for commercial exploitation of the selected genotype.en_US
dc.description.sponsorshipTUBITAK-The Scientific and Technical Research Council of Turkey [KBAG-110T941]; Dicle University [DUBAP-11-FF-81]en_US
dc.description.sponsorshipThis study was funded by Grant #KBAG-110T941 from TUBITAK-The Scientific and Technical Research Council of Turkey, and Dicle University, The Scientific Research Projects # DUBAP-11-FF-81. The authors are also grateful to Metin Gemici for providing the seeds of P. lentiscus L. We are also grateful to Ibrahim Tunik for the language revision of the manuscript.en_US
dc.identifier.doi10.1007/s10725-014-9933-9
dc.identifier.endpage88en_US
dc.identifier.issn0167-6903
dc.identifier.issn1573-5087
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84939893477
dc.identifier.scopusqualityQ1
dc.identifier.startpage75en_US
dc.identifier.urihttps://doi.org/10.1007/s10725-014-9933-9
dc.identifier.urihttps://hdl.handle.net/11468/14664
dc.identifier.volume75en_US
dc.identifier.wosWOS:000349974200008
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofPlant Growth Regulation
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectClonal Micropropagationen_US
dc.subjectGenetic Fidelityen_US
dc.subjectLentisken_US
dc.subjectMastic Treeen_US
dc.subjectPcr-Based Markeren_US
dc.titleClonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markersen_US
dc.titleClonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers
dc.typeArticleen_US

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