Early pregnancy diagnosis using a commercial ELISA test based on pregnancy-associated glycoproteins in Holstein-Friesian heifers and lactating cows

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dc.contributor.authorKaya, Mehmet Salih
dc.contributor.authorKose, Mehmet
dc.contributor.authorBozkaya, Faruk
dc.contributor.authorMutlu, Hasan
dc.contributor.authorUcar, Eyyup Hakan
dc.contributor.authorAtli, Mehmet Osman
dc.date.accessioned2024-04-24T17:21:11Z
dc.date.available2024-04-24T17:21:11Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn this study, we aimed to investigate the efficacy of a commercial ELISA test kit for detecting pregnancy-associated glycoproteins (PAGs) in peripheral blood for early pregnancy diagnosis and compare plasma PAG levels during early pregnancy in both Holstein-Friesian heifers and lactating cows. A total of 231 plasma samples were collected from heifers and lactating cows on days 25, 28, and 32 after insemination. Pregnancies were confirmed 30 days after the collection of plasma samples. Plasma PAG levels were measured using a commercial ELISA test kit for diagnosing bovine pregnancy. The effects of examination date and animal status (heifers vs. lactating cows) on PAG levels in heifers and lactating cows were analyzed using two-way ANOVA. Plasma PAG levels were significantly higher in heifers than in lactating cows; levels also increased significantly with the date of examination, i.e. days 25, 28, and 32 for heifers (P < 0.001), but not for lactating cows (P > 0.05). Although the sensitivity, specificity, and accuracy of pregnancy diagnosis using the ELISA test were acceptable in both groups, the performance of the test was superior in pregnant heifers compared to lactating cows.en_US
dc.identifier.doi10.3906/vet-1602-41
dc.identifier.endpage699en_US
dc.identifier.issn1300-0128
dc.identifier.issue6en_US
dc.identifier.startpage694en_US
dc.identifier.trdizinid213832
dc.identifier.urihttps://doi.org/10.3906/vet-1602-41
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/213832
dc.identifier.urihttps://hdl.handle.net/11468/19398
dc.identifier.volume40en_US
dc.identifier.wosWOS:000391716100003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPregnancy-Associated Glycoproteinsen_US
dc.subjectEarly Pregnancy Diagnosisen_US
dc.subjectLactating Cowsen_US
dc.subjectHeifersen_US
dc.subjectElisaen_US
dc.titleEarly pregnancy diagnosis using a commercial ELISA test based on pregnancy-associated glycoproteins in Holstein-Friesian heifers and lactating cowsen_US
dc.titleEarly pregnancy diagnosis using a commercial ELISA test based on pregnancy-associated glycoproteins in Holstein-Friesian heifers and lactating cows
dc.typeArticleen_US

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