Role of matrix metalloproteinase-9 in the basement membrane destruction of superficial urothelial carcinomas

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dc.contributor.authorÖzdemir, E
dc.contributor.authorKakehi, Y
dc.contributor.authorOkuno, H
dc.contributor.authorYoshida, O
dc.date.accessioned2024-04-24T16:18:41Z
dc.date.available2024-04-24T16:18:41Z
dc.date.issued1999
dc.departmentDicle Üniversitesien_US
dc.description.abstractPurpose: This study was conducted to clarify which matrix metalloproteinases (MMPs) play a key role in destruction of the underlying basement membrane (BM) of superficial urothelial carcinomas. Urine concentrations of MMP-9 and tissue inhibitors of matrix metalloproteinase-l (TIMP-1) were also measured. Materials and Methods: Overexpression of MMP-1, MMP-2 and MMP-9 was analyzed immunohistochemically in 60 patients with transitional cell carcinomas of the urothelium (41 were pTa or pis, 19 were pT(1-4)), and compared them with type IV collagen expression in tumor BM. In 33 of them, urine concentrations of MMP-9 and TIMP-1 were measured by one-step sandwich enzyme immunoassay. Results: Positive expression of MMP-1, MMP-2 and MMP-9 was found in 53%, 17%, and 65% of tumors, respectively. Only MMP-9 expression rates were increased with grades and stages (p = 0.03). In pTa and pis tumors, type IV collagen expression was reduced in 17 of 26 (65.4%), and it was associated with positive MMP-9 expression (p = 0.0283). MMP-9 was detected in all urine samples of urothelial cancer patients, while urine TIMP-1 was detectable in 18 of 33 patients. In 16 healthy volunteers, both of them were below detectable levels, Balance between urinary MMP-9 and TIMP-1 were particularly kept in superficial urothelial carcinomas with intact tumor BM. Tumor BM: status, however, was not associated with urinary MMP-9 or TIMP-1 levels. Conclusions: These results suggest that MMP-9 plays a key role in the invasion step of superficial urothelial carcinomas. Detection of urinary MMP-9 may become a new, non-invasive mean for the diagnosis of urothelial carcinomas.en_US
dc.identifier.doi10.1016/S0022-5347(01)61684-7
dc.identifier.endpage1363en_US
dc.identifier.issn0022-5347
dc.identifier.issue4en_US
dc.identifier.pmid10081908
dc.identifier.startpage1359en_US
dc.identifier.urihttps://doi.org/10.1016/S0022-5347(01)61684-7
dc.identifier.urihttps://hdl.handle.net/11468/16233
dc.identifier.volume161en_US
dc.identifier.wosWOS:000079014000094
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofJournal of Urology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectSuperficial Urothelial Carcinomasen_US
dc.subjectMatrix Metalloproteinasesen_US
dc.subjectTissue Inhibitors Of Matrix Metalloproteinase-1en_US
dc.subjectType Iv Collagenen_US
dc.titleRole of matrix metalloproteinase-9 in the basement membrane destruction of superficial urothelial carcinomasen_US
dc.titleRole of matrix metalloproteinase-9 in the basement membrane destruction of superficial urothelial carcinomas
dc.typeArticleen_US

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