Method validation of 15 phytochemicals inHypericum lysimachioidesvar.spathulatumby LC-MS/MS, and fatty acid, essential oil, and aroma profiles with biological activities

Basit Öğe Kaydı
dc.contributor.authorAkdeniz, Mehmet
dc.contributor.authorYilmaz, Mustafa Abdullah
dc.contributor.authorErtas, Abdulselam
dc.contributor.authorYener, Ismail
dc.contributor.authorFirat, Mehmet
dc.contributor.authorAydin, Firat
dc.contributor.authorKolak, Ufuk
dc.date.accessioned2024-04-24T16:02:31Z
dc.date.available2024-04-24T16:02:31Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim of the present study was to develop and validate a LC-MS/MS method for quantification of 15 phytochemicals inHypericumspecies. The developed method was fully validated in terms of repeatability (inter-day and intra-day precision), limits of detection and quantification, linearity, recovery and relative standard uncertainty. The developed and validated LC-MS/MS method was applied to determine 15 phytochemicals in the ethanol extracts ofH. lysimachioidesvar.spathulatumaerial parts (HLS-A) and roots (HLS-R). Hyperoside was found to be the major compound in HLS-A and HLS-R ethanol extracts (16,560.3 and 3561.6 mu g analyte/g extract, respectively). According to the results of GC-MS analyses,cis-13,16-docosadienoic acid (35.0%), caryophyllene oxide (24.33%) and undecane (28.21%) were determined as the major components in fatty acid, essential oil and aroma compositions ofH. lysimachioidesvar.spathulatum, respectively. HLS-A and HLS-R ethanol extracts showed moderate activity in ABTS cation radical decolorization assay. The major components (hyperoside, astragalin, and quercetin) present in HLS-A and HLS-R ethanol extracts were found to have also the highest antioxidant effect in ABTS cation radical scavenging method. The extracts, main constituents in the extracts and essential oil had no toxic-cytotoxic potential against PDF, MCF-7 and HT-29 cell lines. HLS-A ethanol extract and essential oil exhibited high butyrylcholinesterase inhibitory activity. Quercetin showed the highest inhibitory effect against acetyl- and butyryl-cholinesterase, urease and tyrosinase among the tested samples. According to biological activity studies,H. lysimachioidesvar.spathulatumand their major components might be promoted as promising sources of natural agents and used in the development of nutraceuticals, functional food ingredients and pharmaceutical industry.en_US
dc.description.sponsorshipDicle University [FEN.15.012, DUBTAM.19.001]en_US
dc.description.sponsorshipThis study was funded by Dicle University. Project Number: FEN.15.012 and DUBTAM.19.001en_US
dc.identifier.doi10.1007/s11694-020-00562-6
dc.identifier.endpage3205en_US
dc.identifier.issn2193-4126
dc.identifier.issn2193-4134
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85088276190en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage3194en_US
dc.identifier.urihttps://doi.org/10.1007/s11694-020-00562-6
dc.identifier.urihttps://hdl.handle.net/11468/14823
dc.identifier.volume14en_US
dc.identifier.wosWOS:000550169000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal of Food Measurement and Characterizationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHypericumen_US
dc.subjectLc-Msen_US
dc.subjectMs Method Validationen_US
dc.subjectFatty Aciden_US
dc.subjectEssential Oilen_US
dc.subjectBiological Activitiesen_US
dc.titleMethod validation of 15 phytochemicals inHypericum lysimachioidesvar.spathulatumby LC-MS/MS, and fatty acid, essential oil, and aroma profiles with biological activitiesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu