Solid state fermentation for production of ?-amylase by a thermotolerant Bacillus subtilis from hot-spring water
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Tarih
2003
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The production of extracellular a-amylase by thermotolerant Bacillus subtilis was studied in solid state fermentation (SSF). The effect of wheat bran (WB) and rice husk (RH) was examined. The appropriate incubation period, moisture level, particle size and inoculum concentration was determined. Maximum yields of 159,520 and 21,760 U g(-1) were achieved by employing WB and RH as substrates in 0.1 M phosphate buffer at pH 7 with 30% initial moisture content at 24 and 48 h. Particle size and inoculum concentration were found to be 1000 pm, 20% and 500 mum, 15% for WB and RH, respectively. Enzyme yield was 7.3-fold higher with WB medium compared with RH. (C) 2002 Elsevier Science Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
B. Subtilis, Solid-State Fermentation, Alpha-Amylase
Kaynak
Process Biochemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
38
Sayı
12
