Effect of Oocyte Diameter on in vitro Embryo Production in Dogs

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dc.contributor.authorEvecen, Mithat
dc.contributor.authorCirit, Umut
dc.contributor.authorDemir, Kamber
dc.contributor.authorKaraman, Elif
dc.contributor.authorBakirer, Gul
dc.contributor.authorHamzaoglu, Asiye Izem
dc.contributor.authorBirler, Sema
dc.date.accessioned2024-04-24T17:33:35Z
dc.date.available2024-04-24T17:33:35Z
dc.date.issued2010
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn vitro embryo production has not been confidently applied to the dog successfully. Up to date, only one blactocyst have been achieved by in vitro culture. Therefore, the aim of the present study was to examine the effects of different oocyte diameters (<= 100 mu m and >100 mu m) on in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC) of immature dog oocytes. The study was performed in two steps. At the first step (experiment I), effects of two different oocyte diameters on IVM of dog oocytes were investigated. The nuclear maturation rates were evaluated by aceto-orcein staining method at the end of the IVM. At the second step (experiment II), in vitro matured oocytes were fertilized with fresh spermatozoa for 24 h and in vitro cultured for 7 d. At the end of the IVC period, embryonic development was assessed by microscopic observation at 24 h intervals and fixed for staining by aceto-orcein staining method after 7 days. In comparison relating in IVM and IVF rates, larger oocytes have higher maturation (P<0.05) and cleavage (P<0.01) rates than the smaller ones. Unfortunately, none of the oocyte was reached to morula or blactocyst stage in both groups. In conclusion, it is demonstrated that the oocyte diameter may be a helpful selection criteria for dog in vitro embryo production.en_US
dc.description.sponsorshipIstanbul University [1145]en_US
dc.description.sponsorshipThe present work was supported by the Research Fund of Istanbul University (Project No: 1145)en_US
dc.identifier.endpage950en_US
dc.identifier.issn1300-6045
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-78149489684
dc.identifier.scopusqualityQ3
dc.identifier.startpage947en_US
dc.identifier.urihttps://hdl.handle.net/11468/20761
dc.identifier.volume16en_US
dc.identifier.wosWOS:000285216200009
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectDogen_US
dc.subjectOocyte Diametersen_US
dc.subjectMaturationen_US
dc.subjectFertilizationen_US
dc.subjectIn Vitroen_US
dc.subjectEmbryoen_US
dc.titleEffect of Oocyte Diameter on in vitro Embryo Production in Dogsen_US
dc.titleEffect of Oocyte Diameter on in vitro Embryo Production in Dogs
dc.typeArticleen_US

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