Antibody purification with protein A attached supermacroporous poly(hydroxyethyl methacrylate) cryogel

Basit Öğe Kaydı
dc.contributor.authorAlkan, Hueseyin
dc.contributor.authorBereli, Nilay
dc.contributor.authorBaysal, Zuebeyde
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-04-24T16:10:54Z
dc.date.available2024-04-24T16:10:54Z
dc.date.issued2009
dc.departmentDicle Üniversitesien_US
dc.description.abstractImmunoglobulin G (IgG) Purification from human plasma with protein A attached supermacroporous poly(hydroxyethyl methacrylate) [PHEMA] cryogel has been studied. PHEMA cryogel was prepared by bulk polymerization which proceeds in aqueous solution of monomer frozen inside a plastic syringe (cryo-polymerization). After thawing, the PHEMA cryogel contains a Continuous matrix having interconnected pores of 10-200 mu m size. Protein was covalently attached onto the PHEMA cryogel via cyanogen bromide (CNBr) activation. The maximum IgG adsorption oil the PHEMA/protein A cryogel Was found to be 83.2 mg/g at pH 7.4 from aqueous Solutions. The non-specific IgG adsorption onto the PHEMA cryogel was about 0.38 mg/g. The macropore size of the cryogel makes it possible to process blood cells without blocking the Column. Higher adsorption capacity was observed from human plasma (Lip to 88.1 mg/g). Adsorbed IgG was eluted using 0.1 M glycine-HCl buffer (pH 3.5) with a purity of 85%. PHEMA-protein A cryogel was used for repetitive adsorption/desorption of IgG without noticeable loss in IgG adsorption capacity after 10 cycles. PHEMA-protein A cryogel showed several advantages Such as simpler preparation procedure, good selectivity for IgG Purification from human plasma and good stability throughout repeated adsorption-desorption cycles. (C) 2009 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorship[DUBAP-07-01-31]en_US
dc.description.sponsorshipWe are greatful to DUBAP-07-01-31 for their financial support.en_US
dc.identifier.doi10.1016/j.bej.2009.03.013
dc.identifier.endpage208en_US
dc.identifier.issn1369-703X
dc.identifier.issn1873-295X
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-67349244928
dc.identifier.scopusqualityQ2
dc.identifier.startpage201en_US
dc.identifier.urihttps://doi.org/10.1016/j.bej.2009.03.013
dc.identifier.urihttps://hdl.handle.net/11468/15178
dc.identifier.volume45en_US
dc.identifier.wosWOS:000274704200005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofBiochemical Engineering Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectProtein Aen_US
dc.subjectCryogelsen_US
dc.subjectPhemaen_US
dc.subjectAntibody Purificationen_US
dc.subjectIggen_US
dc.titleAntibody purification with protein A attached supermacroporous poly(hydroxyethyl methacrylate) cryogelen_US
dc.titleAntibody purification with protein A attached supermacroporous poly(hydroxyethyl methacrylate) cryogel
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu