LC-MS/MS analyses of Ziziphora clinopodioides Lam. from Turkey: Antioxidant, anticholinesterase, antimicrobial and, anticancer activities

Basit Öğe Kaydı
dc.contributor.authorOzkan, Esra Eroglu
dc.contributor.authorBoga, Mehmet
dc.contributor.authorYilmaz, Mustafa Abdullah
dc.contributor.authorKara, Emel Mataraci
dc.contributor.authorYesil, Yeter
dc.date.accessioned2024-04-24T17:20:22Z
dc.date.available2024-04-24T17:20:22Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground and Aims: Ziziphora clinopodioides is one of the wild edible species used in Turkish folk medicine. The study was aimed to investigate the phenolic compounds of the extracts as well as the antioxidant, enzyme inhibitory, antimicrobial activities, and anticancer potential. Methods: LC-MS/MS was used to determine phenolic compounds; antioxidant potential was evaluated by using radical scavenging assays; enzyme inhibition assays were used for anticholinesterase, tyrosinase, and urease activities; antimicrobial activities were performed by microdilution method; and XTT bioassay was used for testing anticancer potential. Results: The LC-MS/MS results indicate that quinic acid (9020.51 +/- 73.97 mu g/g; 14721.04 +/- 120.71 mu g/g, respectively) is the major compound, malic acid (1972.95 +/- 22.29 mu g/g; 2179.04 +/- 24.62 mu g/g, respectively) and rhoifolin (1044.74 +/- 98.31 mu g/g; 3593.31 +/- 338.13 mu g/g, respectively) are the abundant compounds in aerial and root extracts. The antioxidant activity results showed that the aerial parts extract has stronger ABTS cation radical and DPPH free radical scavenging activity than the root extract with 40.90 +/- 0.19 mu g/mL, and 94.27 +/- 0.64 mu g/mL IC50 values, respectively. The extracts showed moderate cupric reducing activity with 1.74 absorbance value at 100 mu g/mL. Only the aerial parts extract exhibited weak tyrosinase inhibition (8.60 +/- 0.87%) compared with kojic acid (95.26 +/- 0.23%) at 200 mu g/mL. No activity was observed in urease and anticholinesterase enzymes. Both extracts exhibited moderate antifungal activity against Candida tropicalis with 39.06 mu g/mL MIC values. The extracts didn't show cytotoxic activity against Renal (A498, UO-31) and Colo (COLO205, KM12) cell lines, also there is no metastatic potential of the extracts against osteosarcoma cell lines (MG63.3, MG63). Conclusion: Z. clinopodioides has rich phytochemical constituents with powerful health benefits, and doesn't have any harmful effect on the body.en_US
dc.description.sponsorshipDicle University Science and Technology Research and Application Center (DUBTAM)en_US
dc.description.sponsorshipThe authors are thankful to Dicle University Science and Technology Research and Application Center (DUBTAM) for its support in this study. They are also thankful to National Cancer Institute, Center for Cancer Research, Molecular Targets Program for its contribution to cytotoxicity assays.en_US
dc.identifier.doi10.26650/IstanbulJPharm.2019.0064
dc.identifier.endpage41en_US
dc.identifier.issn2587-2087
dc.identifier.issue1en_US
dc.identifier.startpage33en_US
dc.identifier.trdizinid379855
dc.identifier.urihttps://doi.org/10.26650/IstanbulJPharm.2019.0064
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/379855
dc.identifier.urihttps://hdl.handle.net/11468/18994
dc.identifier.volume50en_US
dc.identifier.wosWOS:000523300100006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.publisherIstanbul Univ, Fac Pharmacyen_US
dc.relation.ispartofIstanbul Journal of Pharmacy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectZiziphora Clinopodioidesen_US
dc.subjectAntioxidanten_US
dc.subjectEnzyme Inhibitionen_US
dc.subjectAntimicrobialen_US
dc.subjectAnticanceren_US
dc.subjectLc-Ms/Msen_US
dc.titleLC-MS/MS analyses of Ziziphora clinopodioides Lam. from Turkey: Antioxidant, anticholinesterase, antimicrobial and, anticancer activitiesen_US
dc.titleLC-MS/MS analyses of Ziziphora clinopodioides Lam. from Turkey: Antioxidant, anticholinesterase, antimicrobial and, anticancer activities
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu