Dissemination of blaOXA-48 like, blaNDM, blaKPC, blaIMP-1, blaVIM genes among carbapenem-resistant Escherichia coli and Klebsiella pneumoniae strains in Southeastern Turkey: first report of Klebsiella pneumoniae co-producing blaOXA-48-like, blaVIM and blaIMP-1 genes

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dc.contributor.authorBilik, Ozge Alkan
dc.contributor.authorBayraktar, Mehmet
dc.contributor.authorOzcan, Nida
dc.contributor.authorGul, Kadri
dc.contributor.authorAkpolat, Nezahat
dc.date.accessioned2024-04-24T17:08:24Z
dc.date.available2024-04-24T17:08:24Z
dc.date.issued2021
dc.departmentDicle Üniversitesien_US
dc.description.abstractIntroduction: Carbapenem-resistant Enterobacterales (CRE) cause serious and life-threatening infections with limited treatment options. The most common causes of carbapenem resistance are carbapenemases. We aimed to determine the most prevalent carbapenemase genes; bla(NDM), bla(KPC), bla(IMP-1), bla(VIM), bla(OXA-48) like genes among Klebsiella pneumoniae and Escherichia coli strains in the southeast region of Turkey. Methods: Eighty-nine isolates (74 K. pneumoniae, 15 E. coli) were included in the study. The isolates were found as carbapenem-resistant by BD Phoenix automated system and Kirby Bauer disk diffusion test. Antibiotic susceptibility testing was performed by BD Phoenix automated system. Combination disc method (CDM) was also carried out as phenotypic method of carbapenemase detection. The presence of bla(NDM), bla(KPC), bla(IMP-1), bla(VIM) and bla(OXA-48)-like genes were investigated by Xpert CARBA-R (Cepheid, USA) multiplex PCR commercial system. Results: Antibiotic resistance rates by Phoenix were 48.3 97.8, 80.9, 96.6, 93.3, 96.6, 97.8, 69.7 and 97.8% for amikacin, aztreonam, cefepime, ceftazidime, ciprofloxacin, levofloxacin, moxifloxacin, trimethoprim sulfamethoxazole and piperacillin-tazobactam, respectively. The bla(OXA-48)-like gene was detected in 65 (73%); bla(NDM) gene in 4 (4.5%); co-production of bla(OXA-48)-like and bla(NDM) in 6 (6.7%); co-production of bla(OXA-48)-like, bla(VIM) and bla(IMP-1) genes in 1(1.2%) isolate. None of the bla(OXA-48)-like, bla(NDM), bla(VIM), bla(IMP-1), bla(KPC) genes were detected in 13 (14.6%) of the isolates. The sensitivity and specificity of CDM were calculated as 80 and 85% respectively. Conclusion: We detected bla(OXA-48)-like gene most frequently in our region. To our knowledge, this is the first report of K. pneumoniae-co-producing bla(OXA-48)-like, bla(VIM) and bla(IMP-1) genes. The coexistence of these genes is alarming and causes both infection control and treatment problems. Effective infection control measures are essential to prevent the spread of antibiotic resistance. Copyright (C) 2021 Wolters Kluwer Health, Inc. All rights reserved.en_US
dc.description.sponsorshipHarran University Scientific Research Projects Coordination Board [16194]en_US
dc.description.sponsorshipThis project was funded by Harran University Scientific Research Projects Coordination Board with project number 16194.en_US
dc.identifier.doi10.1097/MRM.0000000000000287
dc.identifier.endpage210en_US
dc.identifier.issn0954-139X
dc.identifier.issn1473-5601
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85123737857
dc.identifier.scopusqualityQ4
dc.identifier.startpage205en_US
dc.identifier.urihttps://doi.org/10.1097/MRM.0000000000000287
dc.identifier.urihttps://hdl.handle.net/11468/17327
dc.identifier.volume32en_US
dc.identifier.wosWOS:000700928400002
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofReviews in Medical Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBla(Imp-1)en_US
dc.subjectBla(Ndm)en_US
dc.subjectBla(Vim)en_US
dc.subjectBla(Oxa-48)en_US
dc.subjectCarbapenemase Co-Producing Klebsiella Pneumoniaeen_US
dc.subjectCarbapenem-Resistant Enterobacteralesen_US
dc.subjectMultiplex Pcren_US
dc.titleDissemination of blaOXA-48 like, blaNDM, blaKPC, blaIMP-1, blaVIM genes among carbapenem-resistant Escherichia coli and Klebsiella pneumoniae strains in Southeastern Turkey: first report of Klebsiella pneumoniae co-producing blaOXA-48-like, blaVIM and blaIMP-1 genesen_US
dc.titleDissemination of blaOXA-48 like, blaNDM, blaKPC, blaIMP-1, blaVIM genes among carbapenem-resistant Escherichia coli and Klebsiella pneumoniae strains in Southeastern Turkey: first report of Klebsiella pneumoniae co-producing blaOXA-48-like, blaVIM and blaIMP-1 genes
dc.typeArticleen_US

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