Carboligation reactivity of benzaldehyde lyase (BAL, EC 4.1.2.38) covalently attached to magnetic nanoparticles

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dc.contributor.authorTural, Bilsen
dc.contributor.authorTuran, Ilke Simsek
dc.contributor.authorTural, Servet
dc.contributor.authorCelebi, Bulent
dc.contributor.authorDemir, Ayhan S.
dc.date.accessioned2024-04-24T16:18:27Z
dc.date.available2024-04-24T16:18:27Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractEpoxy-functionalized Fe3O4-SiO2 core-shell magnetic nanoparticles (epoxy-M-support) were prepared by modification with glycidyloxypropyltrimethoxysilane (GPTMS) and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), and fourier transform infrared spectroscopy (FTIR) methods. Pure histidine-tagged recombinant benzaldehydelyase (BAL, EC 4.1.238) was efficiently immobilized onto the epoxy-M-support with covalent binding. An immobilized BAL epoxy-M-support system was tested to catalyze the self and cross condensation reactions of aldehydes, and the kinetic resolution of racemic acyloins. The acyloin products were obtained in high yield and with high enantiomeric excesses (>= 98% ee). The carboligation reactivity of the immobilized enzyme was comparable to that of free enzyme-catalyzed reactions. The covalent immobilization offers high enzyme activity and stability (at least 5 repeats without losing its activity). (C) 2013 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipMiddle East Technical University; Dicle University Research Fund (DUBAP) [10-ZEF-128, 10-ZEF-129]; Scientific and Technological Research Council of Turkey (TUBITAK) [109T497]; COST Action [CM0701]en_US
dc.description.sponsorshipWe thank for financial support the Middle East Technical University, Dicle University Research Fund (DUBAP, Project No. 10-ZEF-128 and Project No. 10-ZEF-129), the Scientific and Technological Research Council of Turkey (TUBITAK, 109T497), COST Action CM0701.en_US
dc.identifier.doi10.1016/j.tetasy.2013.01.022
dc.identifier.endpage268en_US
dc.identifier.issn0957-4166
dc.identifier.issue5-6en_US
dc.identifier.scopus2-s2.0-84875132222
dc.identifier.scopusqualityN/A
dc.identifier.startpage260en_US
dc.identifier.urihttps://doi.org/10.1016/j.tetasy.2013.01.022
dc.identifier.urihttps://hdl.handle.net/11468/16108
dc.identifier.volume24en_US
dc.identifier.wosWOS:000316843000004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofTetrahedron-Asymmetry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[No Keyword]en_US
dc.titleCarboligation reactivity of benzaldehyde lyase (BAL, EC 4.1.2.38) covalently attached to magnetic nanoparticlesen_US
dc.titleCarboligation reactivity of benzaldehyde lyase (BAL, EC 4.1.2.38) covalently attached to magnetic nanoparticles
dc.typeArticleen_US

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