Direct plant regeneration from in vitro-derived leaf explants of Hypericum spectabile, a medicinal plant

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dc.contributor.authorAkbas, Filiz
dc.contributor.authorIsikalan, Cigdem
dc.contributor.authorNamli, Sureyya
dc.contributor.authorKarakus, Pinar
dc.contributor.authorBasaran, Davut
dc.date.accessioned2024-04-24T17:33:29Z
dc.date.available2024-04-24T17:33:29Z
dc.date.issued2011
dc.departmentDicle Üniversitesien_US
dc.description.abstractAn efficient and reproducible procedure is described for direct plant regeneration using in vitro regenerated leaf explants of Hypericum spectabile. The leaf explants were cultured on MS media supplemented with six different concentrations (0.25, 0.5, 1.0, 1.5, 2.0, 2.5 mg/L) of BAP and kinetin seperately. All of the BAP concentration, shoot regeneration occurred directly without callus formation, but the number of shoots changed, depending on the different concentrations of BAP. The highest and the lowest number of shoots were obtained on the medium supplemented with 1.0 and 2.5 mg/L of BAP (90 to 50%, respectively). In the present study, the medium containing 0.25, 0.5 and 2.5 mg/L Kin did not promote adventitious shoots formation. Among concentrations of Kin, the best results in terms of both shoots number and morphogenic properties were obtained from MS medium supplemented with 1.0 mg/L Kin (60%). In vitro regenerated plants induced roots on half-strength MS medium containing all the concentrations (0.25, 0.5, 1.0, 2.0 mg/L) of IAA. However, the medium supplemented with 0.5 mg/L IAA (100%) was found to be optimum for inducing root. Rooted 5- to 6-week-old in vitro regenerated plants were transferred into a greenhouse for acclimatization.en_US
dc.identifier.endpage2181en_US
dc.identifier.issn1996-0875
dc.identifier.issue11en_US
dc.identifier.scopus2-s2.0-79959830741
dc.identifier.scopusqualityN/A
dc.identifier.startpage2175en_US
dc.identifier.urihttps://hdl.handle.net/11468/20713
dc.identifier.volume5en_US
dc.identifier.wosWOS:000297457600009
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherAcademic Journalsen_US
dc.relation.ispartofJournal of Medicinal Plants Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectHypericum Spectabileen_US
dc.subjectDirect Regenerationen_US
dc.subjectLeaf Explanten_US
dc.subjectIn Vitroen_US
dc.subjectPgrsen_US
dc.titleDirect plant regeneration from in vitro-derived leaf explants of Hypericum spectabile, a medicinal planten_US
dc.titleDirect plant regeneration from in vitro-derived leaf explants of Hypericum spectabile, a medicinal plant
dc.typeArticleen_US

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