Use of poly(HEMA-MAH)-$Cu^{2+}$ Microbeads for ?-Amylase Immobilization
| dc.contributor.author | Baysal, Zübeyde | |
| dc.contributor.author | Ayla, Ayşegül | |
| dc.date.accessioned | 2024-04-24T19:05:23Z | |
| dc.date.available | 2024-04-24T19:05:23Z | |
| dc.date.issued | 2014 | |
| dc.department | Dicle Üniversitesi | en_US |
| dc.description.abstract | In this article poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-histidin methylester) [P(HEMA-MAH)] microbeads were prepared by suspension polymerization of 2-hydroxyethyl methacrylate (HEMA) and N-methacryloyl-(l)-histidine methyl ester (MAH). Some properties of the p(HEMA-MAH) microbeads were determined by using scanning electron microscopy (SEM) and swelling tests. $Cu^{2+}$ ions were chelated on the p(HEMA-MAH) microbeads, then these microbeads were used in the immobilization of ?-amylase in batch system. The maximum ?-amylase adsorption capacity of the p(HEMA-MAH)-$Cu^{2+}$ beads was found to be 14.88 mg/g at pH 6.0. The optimum temperatures for the immobilized and free enzyme were determined to be 30ºC and 25 ºC, respectively. Kinetic parameters $(K_m, V_{max})$ of the immobilized enzyme were also determined and compared with those of free enzyme. Reuse studies showed that the immobilized enzyme could reuse six times while retaining 76% of its activity. | en_US |
| dc.identifier.endpage | 330 | en_US |
| dc.identifier.issn | 1303-5002 | |
| dc.identifier.issn | 2687-475X | |
| dc.identifier.issue | 3 | en_US |
| dc.identifier.startpage | 323 | en_US |
| dc.identifier.trdizinid | TRDizinIdYok | |
| dc.identifier.uri | https://hdl.handle.net/11468/27211 | |
| dc.identifier.volume | 42 | en_US |
| dc.indekslendigikaynak | TR-Dizin | |
| dc.language.iso | en | en_US |
| dc.relation.ispartof | Hacettepe Journal of Biology and Chemistry | |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.title | Use of poly(HEMA-MAH)-$Cu^{2+}$ Microbeads for ?-Amylase Immobilization | en_US |
| dc.title | Use of poly(HEMA-MAH)-$Cu^{2+}$ Microbeads for ?-Amylase Immobilization | |
| dc.type | Article | en_US |
