Use of poly(HEMA-MAH)-$Cu^{2+}$ Microbeads for ?-Amylase Immobilization
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Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this article poly(2-hydroxyethyl methacrylate-N-methacryloyl-(l)-histidin methylester) [P(HEMA-MAH)] microbeads were prepared by suspension polymerization of 2-hydroxyethyl methacrylate (HEMA) and N-methacryloyl-(l)-histidine methyl ester (MAH). Some properties of the p(HEMA-MAH) microbeads were determined by using scanning electron microscopy (SEM) and swelling tests. $Cu^{2+}$ ions were chelated on the p(HEMA-MAH) microbeads, then these microbeads were used in the immobilization of ?-amylase in batch system. The maximum ?-amylase adsorption capacity of the p(HEMA-MAH)-$Cu^{2+}$ beads was found to be 14.88 mg/g at pH 6.0. The optimum temperatures for the immobilized and free enzyme were determined to be 30ºC and 25 ºC, respectively. Kinetic parameters $(K_m, V_{max})$ of the immobilized enzyme were also determined and compared with those of free enzyme. Reuse studies showed that the immobilized enzyme could reuse six times while retaining 76% of its activity.
Açıklama
Anahtar Kelimeler
Kaynak
Hacettepe Journal of Biology and Chemistry
WoS Q Değeri
Scopus Q Değeri
Cilt
42
Sayı
3
