A comprehensive study on chemical and biological investigation of thymus brachychilus jalas: A rich source of ursolic and oleanolic acids

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dc.contributor.authorAkdeniz, Mehmet
dc.contributor.authorYiğitkan, Serkan
dc.contributor.authorYılmaz, Mustafa Abdullah
dc.contributor.authorYener, İsmail
dc.contributor.authorVarhan Oral, Elif
dc.contributor.authorFırat, Mehmet
dc.contributor.authorOrhan, İlkay Erdoğan
dc.date.accessioned2024-04-24T16:24:16Z
dc.date.available2024-04-24T16:24:16Z
dc.date.issued2023
dc.departmentDicle Üniversitesi, Eczacılık Fakültesi, Eczacılık Meslek Bilimleri Bölümüen_US
dc.description.abstractThe significance of Thymus species in the scientific community is growing steadily due to their extensive utilization in traditional medicine, food industry, and pharmaceutical sector, owing to their abundance in essential oil and phytochemical content, rendering them commercially significant species. The current work focuses on conducting a comprehensive analysis of the ethanol extract and essential oil derived from the root and aerial portions of Thymus brachychilus Jalas, an endemic species that has not been previously investigated. Additionally, a novel GC-MS (gas chromatography-mass spectrometry) technique was developed to quantify the levels of triterpenoids, which are frequently found in many plants, particularly those belonging to the Lamiaceae family. The approach was then used to assess the triterpenoid content of the species. While the phenolic content of the species was determined by LC-MS/MS (liquid chromatography-tandem mass spectrometry), the chemical composition of triterpenoid, essential oil and flavor (aroma) of the plant was determined by GC-MS. Eucalyptol was the primary ingredient in both the essential oil and the flavor, accounting for 11.05% and 12.35%, respectively. In the DPPH and ABTS radical scavenging techniques, the root ethanol extract exhibited the greatest antioxidant activity with IC50 values of 26.70 +/- 0.23 and 19.16 +/- 0.11 mu g/mL, respectively. There was a high level of urease (59.54 +/- 1.67% at 100 mu g/mL) inhibitory activity observed in the root ethanol extract in enzyme inhibition assays, as well as angiotensin (94.80 +/- 0.56%), elastase (40.19 +/- 0.39%), and collagenase (48.26 +/- 0.12%) inhibition in the aerial ethanol extract. Moreover, the MCF-7 cell line for breast cancer had a strong cytotoxic impact when exposed to the essential oil of the species (vitality%: 1.45 +/- 0.01 at 200 mu g/mL. The LC-MS/MS and GC-MS studies revealed that the roots had a significant concentration of rosmarinic acid (15,801 mu g analyte/g extract), but the aerial portions had a substantial quantity of ursolic acid (152,374 mu g analyte/g extract). The plant exhibits potential in the food, cosmetic, and pharmaceutical sectors on account of its elevated levels of rosmarinic and ursolic acid, superior antioxidant capacity, and angiotensin and urease inhibitory effect.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit of Dicle University; Scientific Research Projects Coordination Unit of Dicle Universityen_US
dc.description.sponsorshipThe authors thank Scientific Research Projects Coordination Unit of Dicle University for their support in this study.en_US
dc.identifier.citationAkdeniz, M., Yiğitkan, S., Yılmaz, M. A., Yener, İ., Oral, E. V., Fırat, M. ve diğerleri. (2023). A comprehensive study on chemical and biological investigation of thymus brachychilus jalas: A rich source of ursolic and oleanolic acids. Analytical Letters, 1-17.
dc.identifier.doi10.1080/00032719.2023.2300737
dc.identifier.issn0003-2719
dc.identifier.issn1532-236X
dc.identifier.scopus2-s2.0-85182204639
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1080/00032719.2023.2300737
dc.identifier.urihttps://hdl.handle.net/11468/16624
dc.identifier.wosWOS:001140961200001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherTaylor & Francis Incen_US
dc.relation.ispartofAnalytical Letters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnti-agingen_US
dc.subjectAntihypertensiveen_US
dc.subjectGas chromatography-mass spectrometry (Gc-Ms)en_US
dc.subjectHigh-performance liquid chromatography-tandem mass spectrometry (Hplc-Ms/Ms)en_US
dc.subjectThymus brachychilusen_US
dc.subjectUrsolic aciden_US
dc.titleA comprehensive study on chemical and biological investigation of thymus brachychilus jalas: A rich source of ursolic and oleanolic acidsen_US
dc.titleA comprehensive study on chemical and biological investigation of thymus brachychilus jalas: A rich source of ursolic and oleanolic acids
dc.typeArticleen_US

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