Optimization and enhanced production of ?-amylase and protease by a newly isolated Bacillus licheniformis ZB-05 under solid-state fermentation
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Tarih
2013
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Eight different agro-residues were tested for alpha-amylase and protease production by using Bacillus licheniformis ZB-05. Among them, rice husk (RH) was proved as the best substrate for two enzymes (alpha-amylase 443 U/g and protease 469,000 U/g). Maximum enzyme production was observed to be 30 % initial moisture, with a growth period of 36 h in 20 and 30 % inoculum volumes for alpha-amylase and protease, respectively. The best enzyme recovery from solid mass was obtained when extracted with tap water. Among the tested various nitrogen sources, 1 % ammonium sulphate followed by 2 % Bacto liver, 2 % ammonium sulphate and 1 % Bacto casaminoacid served as the best inorganic and organic nitrogen sources for alpha-amylase and protease production, respectively. As additional carbon sources, 2 % soluble starch enhanced alpha-amylase production, while 1 % maltose enhanced protease production.
Açıklama
Anahtar Kelimeler
Alpha-Amylase, Agricultural Waste, Bacillus Licheniformis, Enzyme Production, Protease, Solid-State Fermentation
Kaynak
Annals of Microbiology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
63
Sayı
1
