Enhanced production of anticancer triterpenoids in optimized Pistacia lentiscus L. callus cultures via methyl jasmonate and silver nitrate elicitation

Basit Öğe Kaydı
dc.authorid0000-0002-1654-7655en_US
dc.authorid0000-0001-5383-4613en_US
dc.authorid0000-0001-5336-974Xen_US
dc.authorid0000-0002-2193-8386en_US
dc.authorid0000-0002-4090-7227en_US
dc.authorid0000-0003-3933-0245en_US
dc.contributor.authorTilkat, Engin
dc.contributor.authorSüzerer, Veysel
dc.contributor.authorAsan, Hilal Surmuş
dc.contributor.authorErtaş, Abdulselam
dc.contributor.authorDemir, Elif
dc.contributor.authorYılmaz, Mustafa Abdullah
dc.contributor.authorHoşer, Ayşe
dc.contributor.authorOnay, Ahmet
dc.date.accessioned2024-03-06T11:40:48Z
dc.date.available2024-03-06T11:40:48Z
dc.date.issued2023en_US
dc.departmentDicle Üniversitesi, Fen Fakültesi, Biyoloji Bölümüen_US
dc.description.abstractPistacia lentiscus L. is a traditional medicinal plant from the Mediterranean region with a long history of use. Elicitor-based strategies have proven effective in enhancing the production of secondary metabolites (SMs) with therapeutic potential in callus cultures. In addition, optimizing callus cultures is crucial for the synthesis and stability of SMs production. In this study, we have successfully established practical and optimized callus cultures from the leaves and roots of the mastic tree. Full-strength Murashige and Skoog (MS) medium gave the highest callus growth, with the combination of 2,4-dichlorophenoxyacetic acid and kinetin (each at 1 mg/l) giving the most favourable results for both explant types (80 % and 84 % callus induction, respectively). Optimal culture conditions were determined to be a sucrose concentration of 15 g/l, a pH of 5.8, a temperature of 25 °C and light intensities of 20 μmol m−2 s−1 for roots and 80 μmol m−2 s−1 for leaves. It is interesting to note that methyl jasmonate (MeJA) positively affected callus biomass, whereas silver nitrate (AgNO3) had the opposite effect. Elicitation with AgNO3 and MeJA significantly enhanced the biosynthesis of anticancer triterpenoids, particularly ursonic acid (UA), ursolic acid (ULA), masticadienolic acid (MDLA), and oleanonic acid (ONNA). Liquid Chromatography-Mass Spectrometry (LC-MS/MS) analysis revealed a remarkable 26.71-fold and 3.4-fold increase in UA content in leaf and root calli treated with AgNO3, respectively. It is noteworthy that ULA, MDLA and ONNA were not present prior to elicitation but were detected after elicitation. The accumulation of anticancer triterpenoids in callus cultures generated from various P. lentiscus L. explants is first demonstrated in our research. In addition, this research demonstrates a structured methodology for the enhancement of the accumulation of anticancer triterpenoids during callus culture.en_US
dc.identifier.citationTilat, E., Süzerer, V., Asan, H. S., Ertaş, A., Yılmaz, M. A., Demir, E. ve diğerleri. (2023). Enhanced production of anticancer triterpenoids in optimized Pistacia lentiscus L. callus cultures via methyl jasmonate and silver nitrate elicitation. South African Journal of Botany, 163, 63-74.en_US
dc.identifier.doi10.1016/j.sajb.2023.10.025
dc.identifier.endpage74en_US
dc.identifier.issn0254-6299
dc.identifier.scopus2-s2.0-85174412224
dc.identifier.scopusqualityQ1
dc.identifier.startpage65en_US
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0254629923006269?via%3Dihub
dc.identifier.urihttps://hdl.handle.net/11468/13530
dc.identifier.volume163en_US
dc.identifier.wosWOS:001102902100001
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAsan, Hilal Surmuş
dc.institutionauthorErtaş, Abdulselam
dc.institutionauthorYılmaz, Mustafa Abdullah
dc.institutionauthorOnay, Ahmet
dc.language.isoenen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofSouth African Journal of Botany
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.relation.tubitakKBAG-114Z842
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnticancer compoundsen_US
dc.subjectBiosynthesis of triterpenoidsen_US
dc.subjectCallus culture optimizationen_US
dc.subjectElicitationen_US
dc.subjectMastic treeen_US
dc.titleEnhanced production of anticancer triterpenoids in optimized Pistacia lentiscus L. callus cultures via methyl jasmonate and silver nitrate elicitationen_US
dc.titleEnhanced production of anticancer triterpenoids in optimized Pistacia lentiscus L. callus cultures via methyl jasmonate and silver nitrate elicitation
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
Enhanced production of anticancer triterpenoids in optimized Pistacia lentiscus L. callus cultures via methyl jasmonate and silver nitrate elicitation.pdf
Boyut:
1.66 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Makale Dosyası
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
[ X ]
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

Biyoloji Bölümü Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
WoS İndeksli Yayınlar Koleksiyonu