The isolation of tetrangomycin from terrestrial Streptomyces sp CAH29: evaluation of antioxidant, anticancer, and anti-MRSA activity

Basit Öğe Kaydı
dc.contributor.authorOzakin, Suleyman
dc.contributor.authorDavis, Robert W.
dc.contributor.authorUmile, Thomas P.
dc.contributor.authorPirinccioglu, Necmettin
dc.contributor.authorKizil, Murat
dc.contributor.authorCelik, Gurbet
dc.contributor.authorSen, Alaattin
dc.date.accessioned2024-04-24T15:59:43Z
dc.date.available2024-04-24T15:59:43Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractA rhizosphere isolate Streptomyces sp. CAH29 was found to possess potent antibacterial and antifungal activity against a variety of test organisms. Based on 16S ribosomal ribonucleic acid sequence homology studies, this strain was found to be similar to Streptomyces stramineus (gene sequence similarity 99 %). The major bioactive metabolite produced by Streptomyces sp. CAH29 isolate was extracted, purified andidentified by nuclear magnetic resonance as tetrangomycin. This known anthraquinone-exhibited antimicrobial activity against Staphylococcus aureus, Streptococcus pyogenes, methicillin resistant Staphylococcus aureus and Candida albicans with inhibition zones of 14, 10, 12 and 8 mm, respectively. Docking results demonstrate that tetrangomycin has a similar mode of action and a comparable docking score to bind to the dehydrosqualene synthase (CrtM) enzyme of methicillin resistant Staphylococcus aureus compared to the current inhibitor. Hence, this suggests that tetrangomycin has a potential to be used as an anti-methicillin resistant Staphylococcus aureus agent. Tetrangomycin also showed moderate free radical scavenging activity with 1,1-diphenyl-2-picryl-hydrazil. Tetrangomycin apparently decreased all of the studied cytokine (pro-inflammatory: interleukin 1B, interleukin 2, tumor necrosis factor and interleukin L6 and anti-inflammatory: interleukin 10) expression levels at IC50 concentrations in A459 (adenocarcinomic human alveolar basal epithelial) and LNCAP (human prostate adenocarcinoma) cell lines. In addition, it reduced Caspase 8 and 3 mRNA levels in LNCAP and A549 cells. This study describes for the first time novel in vitro immunosuppressive function of tetrangomycin by reducing the transcription of cytokine genes.en_US
dc.description.sponsorshipScientific and Technical Research Council of Turkey [TBAG 109T843]; Dicle University Research Foundation [DUBAP-09-FF-45]; United States National Science Foundation [DEB-1136662]en_US
dc.description.sponsorshipThis study was supported by the Scientific and Technical Research Council of Turkey (TBAG 109T843) and Dicle University Research Foundation (DUBAP-09-FF-45) as well as the United States National Science Foundation (DEB-1136662). The authors would like to thank the staff of the Princeton University NMR and Mass Spectrometry facilities for experimental support.en_US
dc.identifier.doi10.1007/s00044-016-1708-6
dc.identifier.endpage2881en_US
dc.identifier.issn1054-2523
dc.identifier.issn1554-8120
dc.identifier.issue12en_US
dc.identifier.scopus2-s2.0-84984914909
dc.identifier.scopusqualityQ1
dc.identifier.startpage2872en_US
dc.identifier.urihttps://doi.org/10.1007/s00044-016-1708-6
dc.identifier.urihttps://hdl.handle.net/11468/14224
dc.identifier.volume25en_US
dc.identifier.wosWOS:000387329600011
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringer Birkhauseren_US
dc.relation.ispartofMedicinal Chemistry Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectStreptomycesen_US
dc.subjectAngucylinesen_US
dc.subjectTetrangomycinen_US
dc.subjectAntimicrobial Activityen_US
dc.subjectAnti-Mrsaen_US
dc.subjectCytotoxic Activityen_US
dc.subjectImmunosuppressive Activityen_US
dc.titleThe isolation of tetrangomycin from terrestrial Streptomyces sp CAH29: evaluation of antioxidant, anticancer, and anti-MRSA activityen_US
dc.titleThe isolation of tetrangomycin from terrestrial Streptomyces sp CAH29: evaluation of antioxidant, anticancer, and anti-MRSA activity
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu