Increased mRNA expression of ADAMTS1 and ADAMTS4 in peripheral blood mononuclear cells of psoriasis patients developed psoriatic arthritis

Basit Öğe Kaydı
dc.authoridNAS, Kemal/0000-0002-5845-0851
dc.contributor.authorIrtegun-kandemir, Sevgi
dc.contributor.authorPektanc-sengul, Gulsum
dc.contributor.authorTekin, Mehmet ali
dc.contributor.authorAn, Isa
dc.contributor.authorNas, Kemal
dc.contributor.authorCevik, Remzi
dc.date.accessioned2025-02-22T14:08:45Z
dc.date.available2025-02-22T14:08:45Z
dc.date.issued2023
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground and purpose: Psoriatic arthritis (PsA) is a chronic inflammatory disease associated with psoriasis (PsO) affecting both skin and joint. ADAMTS (A disintegrin-like and metalloproteinase domain with thrombospondin-1 repeats) is a large family of proteoglycanase enzymes and the expression levels of ADAMTS proteases are upregulated in arthritis. In this study, we aimed to determine mRNA expression levels of ADAMTS1, ADAMTS4 and ADAMTS5 and identifying the key signaling pathways involved in the regulation of these proteases in peripheral blood mononuclear cells (PBMCs) of patients with PsO who later developed PsA. Materials and methods: 25 PsA patients, 25 PsO patients and 25 healthy individuals were included in this study. PBMCs were isolated from venous blood and mRNA expression levels of ADAMTS1, ADAMTS4 and ADAMTS5 were measured through Real-time quantitative PCR (RTqPCR). Results: mRNA expression levels of ADAMTS1 and ADAMTS4 were found to be increased in PsA patients compared with control and PsO patients. In response to TNF- a stimulation, the expression of ADAMTS1 in PsA patients was determined to be reduced in a Erk1/2 activity dependent manner, whereas p38 and JNK activities were shown to induce the expression of ADAMTS4 in PsA patients. The reduced ADAMTS1 expression in PsA patients induced by IL -1 b stimulation was revealed to be dependent on NFkB activity. Conclusions: mRNA expressions of ADAMTS1 and ADAMTS4 regulated by MAPKs and NFkB were increased in PBMCs of PsA patients. This study supports the hypothesis that ADAMTS1 and ADAMTS4 mRNA level might be diagnostic markers for identifying psoriatic patients who are more likely to develop PsA and a future drug target for PsA treatment.en_US
dc.description.sponsorshipTUBITAK (Scientific and Technical Research Council of Turkey) [214S024]en_US
dc.description.sponsorshipWe thank Bilal Sula for helping with some of the patient samples. This study was supported by the TUBITAK (Scientific and Technical Research Council of Turkey) under the grant 214S024.en_US
dc.identifier.doi10.18054/pb.v125i3-4.25616
dc.identifier.endpage222en_US
dc.identifier.issn0031-5362
dc.identifier.issue3-4en_US
dc.identifier.scopus2-s2.0-85197615169en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage213en_US
dc.identifier.urihttps://doi.org/10.18054/pb.v125i3-4.25616
dc.identifier.urihttps://hdl.handle.net/11468/29623
dc.identifier.volume125en_US
dc.identifier.wosWOS:001265021800008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherPeriodicum Biologorumen_US
dc.relation.ispartofPeriodicum Biologorumen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.snmzKA_WOS_20250222
dc.subjectpsoriatic arthritisen_US
dc.subjectpsoriasisen_US
dc.subjectADAMTS1en_US
dc.subjectADAMTS4en_US
dc.subjectADAMTS5en_US
dc.titleIncreased mRNA expression of ADAMTS1 and ADAMTS4 in peripheral blood mononuclear cells of psoriasis patients developed psoriatic arthritisen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu