The effects of oviductal cell co-culture and different gas mixtures on the development of bovine embryos in vitro

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dc.contributor.authorOzdas, Ozen Banu
dc.contributor.authorCirit, Umut
dc.contributor.authorBaran, Alper
dc.contributor.authorKasikci, Guven
dc.date.accessioned2024-04-24T17:21:11Z
dc.date.available2024-04-24T17:21:11Z
dc.date.issued2012
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe objective of the current study was to investigate the effects of the addition of oviduct co-culture to synthetic oviduct fluid (SOF) and Menezo B2 media used in an in vitro study of cows, and the effects of different gas atmospheres (5% CO2 or 5% CO2, 5% O-2, and 90% N-2) on in vitro cultures. Oocytes obtained through aspiration from the ovaries of slaughtered Holstein cows were washed and cultured within a TCM-199 maturation medium at 38.8 degrees C for 23 h. Then the matured oocytes and thawed semen, prepared in accordance with the swim-up method, were incubated for fertilization within in vitro fertilization (IVF)-Tyrode's albumin lactate pyruvate (TALP) medium with an atmosphere including the combination of 5% CO2, 5% O-2, and 90% N-2 gas, at 38.8 degrees C for 18-24 h. Fertilized oocytes were distributed into 3 main groups: Group I: B2 (5% CO2), Group II: B2 medium (5% CO2, 5% O-2, and 90% N-2), and Group III: SOF (5% CO2, 5% O-2, and 90% N-2), and each group was divided into 2 subgroups, with and without oviduct co-culture cells, and were cultured for 9 days. The percentage of embryos that reached the blastocyst stage was 34.0%, 20.0%, and 32.3% in the co-culture group and 26.7% and 23.3% in the without co-culture group, respectively. Group III showed the highest development of expanded blastocyst stage (P < 0.01). In conclusion, the addition of co-culture to B2 (5% CO2 atmosphere) and SOF (5% CO2, 5% O-2, and 90% N-2 atmosphere) media increased the rate of transferable embryos.en_US
dc.identifier.doi10.3906/vet-1104-25
dc.identifier.endpage366en_US
dc.identifier.issn1300-0128
dc.identifier.issue4en_US
dc.identifier.startpage362en_US
dc.identifier.trdizinid134889
dc.identifier.urihttps://doi.org/10.3906/vet-1104-25
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/134889
dc.identifier.urihttps://hdl.handle.net/11468/19397
dc.identifier.volume36en_US
dc.identifier.wosWOS:000307190100007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCattleen_US
dc.subjectEmbryoen_US
dc.subjectOviduct Co-Cultureen_US
dc.subjectIn Vitroen_US
dc.subjectGas Mixtureen_US
dc.titleThe effects of oviductal cell co-culture and different gas mixtures on the development of bovine embryos in vitroen_US
dc.titleThe effects of oviductal cell co-culture and different gas mixtures on the development of bovine embryos in vitro
dc.typeArticleen_US

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