Direct shoot organogenesis from in vitro-derived mature leaf explants of pistachio

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dc.contributor.authorTilkat, E.
dc.contributor.authorOnay, A.
dc.date.accessioned2024-04-24T16:02:29Z
dc.date.available2024-04-24T16:02:29Z
dc.date.issued2009
dc.departmentDicle Üniversitesien_US
dc.description.abstractAn efficient system was developed for direct plant regeneration from in vitro-derived leaf explants of Pistacia vera L. cv. Siirt. The in vitro procedure involved four steps that included (1) induction of shoot initials from the regenerated mature leaf tissue, (2) regeneration and elongation of shoots from the shoot initials, (3) rooting of the shoots, and (4) acclimatization of the plantlets. The induction of shoot initials was achieved on an agarified Murashige and Skoog (MS) medium with Gamborg vitamins supplemented in different concentrations of benzylaminopurine (BA) and indole-3-acetic acid (IAA). The best medium for shoot induction was a MS medium with 1 mgl(-1) IAA and 2 mgl(-1) BA. Numerous shoot primordia developed within 2-3 wk on the leaf margin and the midrib region, without any callus phase. In the second step, the shoot clumps were separated from the leaf explants and transferred to a MS medium supplemented with 1 mgl(-1) BA, resulting in a differentiation of the shoot initials into well-developed shoots. The elongated shoots (> 3 cm long) were rooted on a full-strength MS basal medium supplemented with 2 mgl(-1) of indole-3-butyric acid in the third stage. Finally, the rooted plants were transferred to soil with an 80% success rate. This protocol was utilized for the in vitro clonal propagation of this important recalcitrant plant species.en_US
dc.description.sponsorshipTUBITAK-The Scientific and Technical Research Council of Turkey [TOVAG-3355]; DUAPK-Dicle University Research Project Council [05-FF-23, 05-FF-61]en_US
dc.description.sponsorshipThe study was funded by grant # TOVAG-3355 from TUBITAK-The Scientific and Technical Research Council of Turkey. The study was also partially funded by a grant from DUAPK-Dicle University Research Project Council (DUAPK)-05-FF-23 and 05-FF-61. We thank the director of the Pistachio Research Institute, Gaziantep, Turkey, for providing plant samples for this work and Linda Thain Ali for linguistically reviewing the manuscript.en_US
dc.identifier.doi10.1007/s11627-008-9168-4
dc.identifier.endpage98en_US
dc.identifier.issn1054-5476
dc.identifier.issn1475-2689
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-59849106510
dc.identifier.scopusqualityQ1
dc.identifier.startpage92en_US
dc.identifier.urihttps://doi.org/10.1007/s11627-008-9168-4
dc.identifier.urihttps://hdl.handle.net/11468/14811
dc.identifier.volume45en_US
dc.identifier.wosWOS:000263138400011
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-Plant
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLeaf Explanten_US
dc.subjectP. Vera L.en_US
dc.subjectPlant Regeneration.en_US
dc.subjectOrganogenesisen_US
dc.titleDirect shoot organogenesis from in vitro-derived mature leaf explants of pistachioen_US
dc.titleDirect shoot organogenesis from in vitro-derived mature leaf explants of pistachio
dc.typeArticleen_US

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