HPLC Separation of Different Groups of Small Polar Compounds on a Novel Amide-Embedded Stationary Phase

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dc.contributor.authorAral, Hayriye
dc.contributor.authorAral, Tarik
dc.contributor.authorCelik, K. Serdar
dc.contributor.authorZiyadanogullari, Berrin
dc.contributor.authorZiyadanogullari, Recep
dc.date.accessioned2024-04-24T16:02:04Z
dc.date.available2024-04-24T16:02:04Z
dc.date.issued2014
dc.departmentDicle Üniversitesien_US
dc.description.abstractRetention behaviors of an amide-embedded silica base stationary phase, which was recently developed by our group, were studied by using six different groups of small polar compounds including phenolic compounds, substituted anilines, chlorinated herbicides, Sudan dyes and some nucleotides and nucleosides in HPLC. The chromatographic behaviors of the prepared stationary phase for these analytes were compared with those of a commercially available reversed-phase column ACE C18 under same conditions. Among the six groups of analytes studied, the amide-silica stationary phase showed enhanced selectivity towards phenolic compounds, substituted anilines, Sudan dyes and herbicides under reversed-phase conditions and satisfactory selectivity towards nucleosides and nucleotides which could not be separated with ACE C18 column under HILIC conditions. Experimental data provided some evidence that functional groups on the stationary phases might have certain degrees of influence on selectivity possibly through secondary interactions with the model compounds. The retentions of the moderately polar compounds such as phenolic acids, anilines and herbicides on the stationary phase are higher than highly polar compounds such as nucleotides and nucleosides due to both the hydrophobic and hydrophilic interactions between the stationary phase and analytes. The quantitative determination of Sudan dyes (I, II, III, and IV) in red chilli peppers was performed. Many red chilli peppers were screened and three of them contained Sudans dyes.en_US
dc.description.sponsorshipResearch Project Council of Dicle University (DUBAP) [10-FF-29]en_US
dc.description.sponsorshipThe Authors gratefully acknowledge the financial support provided by the Research Project Council of Dicle University (DUBAP) (Project No: 10-FF-29).en_US
dc.identifier.doi10.1007/s10337-014-2678-9
dc.identifier.endpage781en_US
dc.identifier.issn0009-5893
dc.identifier.issn1612-1112
dc.identifier.issue11-12en_US
dc.identifier.scopus2-s2.0-84902283662
dc.identifier.scopusqualityQ3
dc.identifier.startpage771en_US
dc.identifier.urihttps://doi.org/10.1007/s10337-014-2678-9
dc.identifier.urihttps://hdl.handle.net/11468/14605
dc.identifier.volume77en_US
dc.identifier.wosWOS:000337158600003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofChromatographia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmide-Embedded Stationary Phaseen_US
dc.subjectPhenolic Compoundsen_US
dc.subjectHerbicidesen_US
dc.subjectAnilinesen_US
dc.subjectSudan Dyesen_US
dc.subjectNucleotides And Nucleosidesen_US
dc.titleHPLC Separation of Different Groups of Small Polar Compounds on a Novel Amide-Embedded Stationary Phaseen_US
dc.titleHPLC Separation of Different Groups of Small Polar Compounds on a Novel Amide-Embedded Stationary Phase
dc.typeArticleen_US

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