Stability-indicating high performance thin layer chromatographic determination of sulfanilamide in human urine

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dc.contributor.authorKilinc, E.
dc.contributor.authorGumgum, B.
dc.contributor.authorHamamci, C.
dc.contributor.authorAydin, F.
dc.date.accessioned2024-04-24T17:11:54Z
dc.date.available2024-04-24T17:11:54Z
dc.date.issued2009
dc.departmentDicle Üniversitesien_US
dc.description.abstractA simple, sensitive, selective, precise and stability-indicating high-performance thin-layer chromatographic (HPTLC) method was developed and applied to human urine for the densitometric determination of sulfanilamide. A mixture of chloroform-ethyl acetate-xylene (2.5: 4.0: 1.0, v/v/v) was used as a mobile phase. The system was found to give compact spots for sulfanilamide (retardation factor, R (f) = 0.21 +/- 0.02). The linear regression analysis data for the calibration plots showed good linear relationship with r (2) = 0.9970 +/- 0.0003 and r (2) = 0.9947 +/- 0.020 within the concentration range of 50-250 ng per spot and 100-1000 ng per spot with respect to peak area, respectively. The limit of detection (LOD) and quantification (LOQ) were 8 and 25 ng per spot, respectively. Sulfanilamide was subjected to acid and alkali hydrolysis, oxidation, dry heat and wet heat treatment. According to the International Conference on Harmonization (ICH) guidelines the method was validated for precision, recovery and robustness. The ultraviolet (UV) spectra of the degradation products which had different spectra from sulfanilamide were also recorded.en_US
dc.description.sponsorshipMehmet Cevik from Republic of Turkey Health Ministry-Refik Saydam Central Hygiene Instituteen_US
dc.description.sponsorshipThe authors especially thank Mehmet Cevik from Republic of Turkey Health Ministry-Refik Saydam Central Hygiene Institute and Dr. Feyyaz Durap from Department of Chemistry, University of Dicle for support.en_US
dc.identifier.doi10.1134/S1061934809070090
dc.identifier.endpage720en_US
dc.identifier.issn1061-9348
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-68149137377
dc.identifier.scopusqualityQ3
dc.identifier.startpage714en_US
dc.identifier.urihttps://doi.org/10.1134/S1061934809070090
dc.identifier.urihttps://hdl.handle.net/11468/17771
dc.identifier.volume64en_US
dc.identifier.wosWOS:000267541300008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherMaik Nauka/Interperiodica/Springeren_US
dc.relation.ispartofJournal of Analytical Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[No Keyword]en_US
dc.titleStability-indicating high performance thin layer chromatographic determination of sulfanilamide in human urineen_US
dc.titleStability-indicating high performance thin layer chromatographic determination of sulfanilamide in human urine
dc.typeArticleen_US

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