An investigation of the relationship between clinical features of amoebiasis and Entamoeba histolytica genotypes

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dc.contributor.authorAraz, Remzi Engin
dc.contributor.authorKoru, Ozgur
dc.contributor.authorTanyuksel, Mehmet
dc.contributor.authorOzekinci, Tuncer
dc.contributor.authorCeylan, Ali
dc.contributor.authorGuclu Kilbas, Hatice Zeynep
dc.contributor.authorCicek, Mutalip
dc.date.accessioned2024-04-24T17:21:08Z
dc.date.available2024-04-24T17:21:08Z
dc.date.issued2012
dc.departmentDicle Üniversitesien_US
dc.description.abstractAim: To determine the presence of Entamoeba histolytica/E. dispar and E. moshkovskii in stool samples, tRNA-based short tandem repeat gene polymorphism in E. histolytica isolates, and the relationship between amoeba load and clinical outcome in studies. Materials and methods: This study involved 840 stools samples of individuals having diarrhea/dysentery and individuals who were asymptomatic by using microscopy, culture, E. histolytica antigen ELISA, and conventional/real-time PCR methods. Results: Of the 840 samples analyzed, 4.3% (36/840), 2.6% (22/840), and 7.4% (62/840) of the stool samples were determined to be positive by E. histolytica antigen ELISA, and real-time PCR for E. histolytica and E. dispar, respectively. Thirty-five of the 62 (56.4%) samples positive for E. dispar and 20 of the 22 (91.0%) samples positive for E. histolytica were from dysenteric individuals as revealed by real-time PCR. Although there was no statistically significant difference in patients with diarrhea, a correlation might be seen between amoeba load and clinical outcome in those infected with E. histolytica, since amoeba load was usually determined 103 copies/mL or higher in patients with diarrhea. In this study, 3 different genotypes were defined in 16 isolates by using 6 loci (A-L, N-K2, D-A, R-R, S-D, and S-TGA-Q). Conclusion: Our results demonstrated that real-time PCR is a useful, reliable, and sensitive method for the determination of E. histolytica in stools and for differentiation from E. dispar. It is suggested that parasite load might affect clinical outcome.en_US
dc.description.sponsorshipTUBITAKen_US
dc.description.sponsorshipThis study was supported by a grant from TUBITAK and was presented as a poster at the 60th ASTMH meeting, 4-8 December 2011, in Philadelphia, USA.en_US
dc.identifier.doi10.3906/sag-1112-23
dc.identifier.endpage1156en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.scopus2-s2.0-84871233488
dc.identifier.scopusqualityQ1
dc.identifier.startpage1147en_US
dc.identifier.urihttps://doi.org/10.3906/sag-1112-23
dc.identifier.urihttps://hdl.handle.net/11468/19378
dc.identifier.volume42en_US
dc.identifier.wosWOS:000312424300001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Medical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEntamoeba Histolyticaen_US
dc.subjectAmoebiasisen_US
dc.subjectReal-Time Pcren_US
dc.subjectTrna Geneen_US
dc.subjectGenotypingen_US
dc.titleAn investigation of the relationship between clinical features of amoebiasis and Entamoeba histolytica genotypesen_US
dc.titleAn investigation of the relationship between clinical features of amoebiasis and Entamoeba histolytica genotypes
dc.typeArticleen_US

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