Determination of serogroup and lytic activities of bacteriophages isolated from phage plaques in Staphylococcus aureus cultures identified from sheep milk with mastitis

dc.authorid0000-0001-9095-6054en_US
dc.authorid0000-0002-0377-2650en_US
dc.authorid0000-0001-7848-3899en_US
dc.authorid0000-0002-5977-7872en_US
dc.contributor.authorGürbilek, Sevil Erdenliğ
dc.contributor.authorArserim, Neval Berrin
dc.contributor.authorTel, Osman Yaşar
dc.contributor.authorSertkaya, Zeynep Tuğçe
dc.contributor.authorKeskin, Oktay
dc.date.accessioned2024-10-17T06:40:27Z
dc.date.available2024-10-17T06:40:27Z
dc.date.issued2024en_US
dc.departmentDicle Üniversitesi, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Mikrobiyoloji Ana Bilim Dalıen_US
dc.description.abstractBackgorund: Bacteriophages are closely related to the evolution and virulence of some important bacterial pathogens. Due to their highly significant roles in pathogenesis and virulence, S. aureus bacteriophages are frequently studied. Bacteriophages are grouped into two main categories depending on their life cycles. There are highly consistently lytic phages (virulent) and temperate phages. This study aimed to isolate bacteriophages and determine their phage serogroups from phage plaques in S. aureus cultures in order to show if they are lytic or lysogenic, the latter plays a major role in horizontal gene transfer. Methods: A total of 234 S. aureus isolates were recovered from milk samples from cases with gangrenous mastitis in sheep. Staphylococcal phages are determined based on the type and serogroup by PCR using specific primers. Result: Our study allowed us to determine serogroups of the isolated bacteriophages. Two phage stock samples included only one serogroup while the others included more than one phage serotypes and needed further purification Fa, L and D serogroups were not determined in the study. Present work revealed that all the isolated phages were temperate phages, which play a highly significant role in horizontal gene transfer.en_US
dc.identifier.citationGürbilek, S. E., Arserim, N. B., Tel, O. Y., Sertkaya, Z. T. ve Keskin, O. (2024). Determination of serogroup and lytic activities of bacteriophages isolated from phage plaques in Staphylococcus aureus cultures identified from sheep milk with mastitis. Indian Journal of Animal Research, 58(8), 1358-1361.en_US
dc.identifier.endpage1361en_US
dc.identifier.issn0367-6722
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-85203083236
dc.identifier.scopusqualityQ3
dc.identifier.startpage1358en_US
dc.identifier.urihttps://arccjournals.com/journal/indian-journal-of-animal-research/BF-1443
dc.identifier.urihttps://hdl.handle.net/11468/28853
dc.identifier.volume58en_US
dc.indekslendigikaynakScopus
dc.institutionauthorArserim, Neval Berrin
dc.language.isoenen_US
dc.publisherAgricultural Research Communication Centreen_US
dc.relation.ispartofIndian Journal of Animal Research
dc.relation.isversionof10.18805/IJAR.BF-1443en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacteriophagesen_US
dc.subjectSheepen_US
dc.subjectStaphyloccocus aureusen_US
dc.titleDetermination of serogroup and lytic activities of bacteriophages isolated from phage plaques in Staphylococcus aureus cultures identified from sheep milk with mastitisen_US
dc.titleDetermination of serogroup and lytic activities of bacteriophages isolated from phage plaques in Staphylococcus aureus cultures identified from sheep milk with mastitis
dc.typeArticleen_US

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