The effects of ionic and non-ionic detergents on Escherichia coli ?-Galactosidase activity.
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Tarih
1998
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Mbr Press Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The effects of several ionic and non-ionic detergents on P-Galactosidase activity at 30 degrees C have been evaluated. The detergent concentrations tested were in the ranges that are already used in most assays to permeablise and reveal proteins from most tissues. The results obtained indicate that the anionic detergent SDS at all concentrations inhibited beta-Galactosidase activity, whereas non-ionic detergents, Triton-X 100 and Tween 20 have no effects on the enzyme activity. The electrophoretic analysis of SDS-exposed enzyme shows two bands corresponding to 170 kD and 116 kD, only the former being active.
Açıklama
Anahtar Kelimeler
[No Keyword]
Kaynak
Biochemical Archives
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
14
Sayı
4
