The effects of ionic and non-ionic detergents on Escherichia coli ?-Galactosidase activity.

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dc.contributor.authorGuven, K
dc.contributor.authorBashan, M
dc.date.accessioned2024-04-24T17:44:21Z
dc.date.available2024-04-24T17:44:21Z
dc.date.issued1998
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe effects of several ionic and non-ionic detergents on P-Galactosidase activity at 30 degrees C have been evaluated. The detergent concentrations tested were in the ranges that are already used in most assays to permeablise and reveal proteins from most tissues. The results obtained indicate that the anionic detergent SDS at all concentrations inhibited beta-Galactosidase activity, whereas non-ionic detergents, Triton-X 100 and Tween 20 have no effects on the enzyme activity. The electrophoretic analysis of SDS-exposed enzyme shows two bands corresponding to 170 kD and 116 kD, only the former being active.en_US
dc.identifier.endpage286en_US
dc.identifier.issn0749-5331
dc.identifier.issue4en_US
dc.identifier.startpage275en_US
dc.identifier.urihttps://hdl.handle.net/11468/21960
dc.identifier.volume14en_US
dc.identifier.wosWOS:000077075800008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoenen_US
dc.publisherMbr Press Incen_US
dc.relation.ispartofBiochemical Archives
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[No Keyword]en_US
dc.titleThe effects of ionic and non-ionic detergents on Escherichia coli ?-Galactosidase activity.en_US
dc.titleThe effects of ionic and non-ionic detergents on Escherichia coli ?-Galactosidase activity.
dc.typeArticleen_US

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